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Fabrication of three-dimensional bioplotted hydrogel scaffolds for islets of Langerhans transplantation
- Source :
- Biofabrication, 7(2):025009. Institute of Physics (IOP), Biofabrication, 7(2):025009. IOP Publishing Ltd., Biofabrication, 7(2), Biofabrication, 7(2). IOP Publishing Ltd.
- Publication Year :
- 2015
-
Abstract
- In clinical islet transplantation, allogeneic islets of Langerhans are transplanted into the portal vein of patients with type 1 diabetes, enabling the restoration of normoglycemia. After intra-hepatic transplantation several factors are involved in the decay in islet mass and function mainly caused by an immediate blood mediated inflammatory response, lack of vascularization, and allo- and autoimmunity. Bioengineered scaffolds can potentially provide an alternative extra-hepatic transplantation site for islets by improving nutrient diffusion and blood supply to the scaffold. This would ultimately result in enhanced islet viability and functionality compared to conventional intra portal transplantation. In this regard, the biomaterial choice, the three-dimensional (3D) shape and scaffold porosity are key parameters for an optimal construct design and, ultimately, transplantation outcome. We used 3D bioplotting for the fabrication of a 3D alginate-based porous scaffold as an extra-hepatic islet delivery system. In 3D-plotted alginate scaffolds the surface to volume ratio, and thus oxygen and nutrient transport, is increased compared to conventional bulk hydrogels. Several alginate mixtures have been tested for INS1E ?-cell viability. Alginate/gelatin mixtures resulted in high plotting performances, and satisfactory handling properties. INS1E ?-cells, human and mouse islets were successfully embedded in 3D-plotted constructs without affecting their morphology and viability, while preventing their aggregation. 3D plotted scaffolds could help in creating an alternative extra-hepatic transplantation site. In contrast to microcapsule embedding, in 3D plotted scaffold islets are confined in one location and blood vessels can grow into the pores of the construct, in closer contact to the embedded tissue. Once revascularization has occurred, the functionality is fully restored upon degradation of the scaffold.
- Subjects :
- Scaffold
type 1 diabetes
Islets of Langerhans Transplantation
Biochemistry
Gelatin
Hydrogel, Polyethylene Glycol Dimethacrylate
Bioplotting
Mice
Tissue engineering
Glucuronic Acid
Insulin Secretion
Insulin
bioplotting
Cells, Cultured
geography.geographical_feature_category
Tissue Scaffolds
Hexuronic Acids
Biomaterial
General Medicine
Islet
Porous scaffold
Type 1 diabetes
tissue engineering
Self-healing hydrogels
islets of Langerhans
Porosity
METIS-311631
Biotechnology
endocrine system
food.ingredient
Materials science
Alginates
Cell Survival
Biomedical Engineering
Bioengineering
Capsules
Mice, Transgenic
Biomaterials
Islets of Langerhans
food
IR-97122
Animals
Humans
geography
Beta cells
Rats
Transplantation
beta cells
Glucose
Microscopy, Fluorescence
Biomedical engineering
Subjects
Details
- Language :
- English
- ISSN :
- 17585082
- Database :
- OpenAIRE
- Journal :
- Biofabrication, 7(2):025009. Institute of Physics (IOP), Biofabrication, 7(2):025009. IOP Publishing Ltd., Biofabrication, 7(2), Biofabrication, 7(2). IOP Publishing Ltd.
- Accession number :
- edsair.doi.dedup.....ce825aa707d2af3d6f7a9a7adbac7794