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Direct roles of the signaling kinase RSK2 in Cdc25C activation during Xenopus oocyte maturation
- Publication Year :
- 2010
- Publisher :
- National Academy of Sciences, 2010.
-
Abstract
- The induction of M phase in eukaryotic cell cycles requires robust activation of Cdc2/cyclin B by Cdc25, which itself is robustly activated by serine/threonine phosphorylations. Although multiple protein kinases that directly activate Cdc25C have been identified, whether the combination of different primary phosphorylations of Cdc25C is sufficient to fully activate Cdc25C has not been determined. By analyzing the GST-Cdc25C phosphorylating activity in Xenopus egg extracts, we previously defined roles of MAPK and Cdc2/cyclin B in partially activating Cdc25C and predicted the presence of another major Cdc25C-activating kinase. In this study, we demonstrate that this missing kinase is RSK2, which phosphorylates three sites in Cdc25C and also partially activates Cdc25C. However, the phosphorylations catalyzed by MAPK, Cdc2, and RSK2 fail to fully activate Cdc25C, suggesting that additional biochemical events are required to fully activate this key cell cycle regulator.
- Subjects :
- MAPK/ERK pathway
Cdc25
Xenopus
Molecular Sequence Data
Cyclin B
Ribosomal Protein S6 Kinases, 90-kDa
Substrate Specificity
Serine
CDC2 Protein Kinase
Animals
cdc25 Phosphatases
Amino Acid Sequence
Amino Acids
Phosphorylation
Cyclin-dependent kinase 1
Multidisciplinary
biology
Kinase
Cell Differentiation
Biological Sciences
biology.organism_classification
Cell biology
Enzyme Activation
Biochemistry
biology.protein
Oocytes
Mitogen-Activated Protein Kinases
Signal Transduction
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....ce6b626dcab7f6ad6e29c0a638d41144