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DFak56 Is a Novel Drosophila melanogaster Focal Adhesion Kinase
- Source :
- Journal of Biological Chemistry. 274:35621-35629
- Publication Year :
- 1999
- Publisher :
- Elsevier BV, 1999.
-
Abstract
- The mammalian focal adhesion kinase (FAK) family of nonreceptor protein-tyrosine kinases have been implicated in controlling a multitude of cellular responses to the engagement of cell surface integrins and G protein-coupled receptors. We describe here aDrosophila melanogaster FAK homologue, DFak56, which maps to band 56D on the right arm of the second chromosome. Full-length DFak56 cDNA encodes a phosphoprotein of 140 kDa, which shares strong sequence similarity not only with mammalian p125FAKbut also with the more recently described mammalian Pyk2 (also known as CAKβ, RAFTK, FAK2, and CADTK) FAK family member. DFak56 has intrinsic tyrosine kinase activity and is phosphorylated on tyrosine in vivo. As is the case for FAK, tyrosine phosphorylation of DFak56 is increased upon plating Drosophila embryo cells on extracellular matrix proteins. In situ hybridization and immunofluorescence staining analysis showed that DFak56 is ubiquitously expressed with particularly high levels within the developing central nervous system. We utilized the UAS-GAL4 expression system to express DFak56 and analyze its function in vivo. Overexpression of DFak56 in the wing imaginal disc results in wing blistering in adults, a phenotype also observed with both position-specific integrin loss of function and position-specific integrin overexpression. Our results imply a role for DFak56 in adhesion-dependent signaling pathways in vivo during D. melanogasterdevelopment.
- Subjects :
- DNA, Complementary
Embryo, Nonmammalian
Molecular Sequence Data
PTK2
Integrin
Biochemistry
Gene Expression Regulation, Enzymologic
Article
Evolution, Molecular
src Homology Domains
Focal adhesion
chemistry.chemical_compound
Animals
Drosophila Proteins
Amino Acid Sequence
Molecular Biology
Phylogeny
Mammals
Sequence Homology, Amino Acid
biology
Cell adhesion molecule
Pupa
Chromosome Mapping
Gene Expression Regulation, Developmental
Tyrosine phosphorylation
Cell Biology
Protein-Tyrosine Kinases
Molecular biology
Recombinant Proteins
Cell biology
Molecular Weight
Drosophila melanogaster
chemistry
Focal Adhesion Kinase 1
Focal Adhesion Protein-Tyrosine Kinases
Larva
biology.protein
Phosphorylation
Signal transduction
Cell Adhesion Molecules
Sequence Alignment
Tyrosine kinase
Subjects
Details
- ISSN :
- 00219258
- Volume :
- 274
- Database :
- OpenAIRE
- Journal :
- Journal of Biological Chemistry
- Accession number :
- edsair.doi.dedup.....ce686793be68cce208c8b4b6dfcc1530