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Comparison of anti-acetylcholine receptor profiles between Chinese cases of adult- and juvenile-onset myasthenia gravis using cell-based assays
- Source :
- Journal of Neuroimmunology. 349:577403
- Publication Year :
- 2020
- Publisher :
- Elsevier BV, 2020.
-
Abstract
- Juvenile-onset myasthenia gravis (JOMG) is a unique clinical subtype in China, featured by a higher prevalence of ocular myasthenia gravis (OMG), higher seronegativity of acetylcholine receptor (AChR) antibodies, and better prognosis than that in adult-onset myasthenia gravis (AOMG). We previously identified low-affinity AChR antibodies in Chinese JOMG patients using cell-based assays (CBAs), indicating a predominantly AChR antibody-positive profile. Here, we further screened AChR antibodies in both Chinese AOMG and JOMG patients by CBAs.We recruited patients with MG who had not received prednisone or immunosuppressive therapies between June 2015 and June 2019, and divided them into AOMG and JOMG subgroups according to their ages at the time of recruitment. Clinical information and blood samples were collected. Serum AChR antibodies were detected by CBAs in HEK293T cells expressing clustered adult and fetal AChRs, as well as by enzyme-linked immunosorbent assays (ELISAs). Differences in AChR antibody profiles between AOMG and JOMG subgroups were determined.A total of 239 patients with MG were enrolled in the present study, including 121 AOMG and 118 JOMG patients. Based on ELISAs, 74.4% of AOMG (90/121) and 59.3% of JOMG (70/118) patients were anti-AChR positive (p = 0.02). However, CBAs yielded equal anti-AChR positivities (p = 0.64), as indicated by 80.2% of AOMG patients (97/121) and 77.1% of JOMG patients (91/118). Furthermore, among AOMG patients, 67.8% (82/121) were positive for both adult and fetal AChR antibodies, 5.8% (7/121) were positive for only adult AChR antibodies, and 6.6% (8/121) were positive for only fetal AChR antibodies, while these rates were 50.8% (60/118), 21.2% (25/118), and 5.1% (6/118), respectively, in JOMG cohorts (p 0.01). Twenty-nine AOMG patients and 10 JOMG patients underwent IgG subclassification of AChR antibodies, which were all confirmed to be predominantly IgG1.The positive rates of AChR antibodies did not differ between Chinese AOMG and JOMG patients, as revealed by CBAs. Furthermore, the screened AChR antibodies were predominantly IgG1 in both AOMG and JOMG patients.
- Subjects :
- Adult
Male
0301 basic medicine
China
Adolescent
Ocular myasthenia
Immunology
Cell
Enzyme-Linked Immunosorbent Assay
Young Adult
03 medical and health sciences
0302 clinical medicine
Prednisone
Myasthenia Gravis
medicine
Humans
Immunology and Allergy
Receptors, Cholinergic
Child
Aged
Autoantibodies
Acetylcholine receptor
Fetus
biology
business.industry
Age Factors
Infant
Middle Aged
medicine.disease
Cell based assays
Myasthenia gravis
HEK293 Cells
030104 developmental biology
medicine.anatomical_structure
Neurology
Child, Preschool
Immunoglobulin G
biology.protein
Female
Neurology (clinical)
Antibody
business
Biomarkers
030217 neurology & neurosurgery
medicine.drug
Subjects
Details
- ISSN :
- 01655728
- Volume :
- 349
- Database :
- OpenAIRE
- Journal :
- Journal of Neuroimmunology
- Accession number :
- edsair.doi.dedup.....ce2a92ed87125a22517f257112579c0c
- Full Text :
- https://doi.org/10.1016/j.jneuroim.2020.577403