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Detection of naphthalene in sea-water by a label-free plasmonic optical fiber biosensor
- Source :
- Talanta (Oxf.) 194 (2019): 289–297. doi:10.1016/j.talanta.2018.10.051, info:cnr-pdr/source/autori:Cennamo, Nunzio; Zeni, Luigi; Ricca, Ezio; Isticato, Rachele; Marzullo, Vincenzo Manuel; Capo, Alessandro; Staiano, Maria; D'Auria, Sabato; Varriale, Antonio/titolo:Detection of naphthalene in sea-water by a label-free plasmonic optical fiber biosensor/doi:10.1016%2Fj.talanta.2018.10.051/rivista:Talanta (Oxf.)/anno:2019/pagina_da:289/pagina_a:297/intervallo_pagine:289–297/volume:194
- Publication Year :
- 2019
-
Abstract
- In this study we developed an optical fiber biosensor able to detect the presence of naphthalene in sea-water. With this aim, we designed and produced an antibody specific for the naphthalene molecule. The capability of the antibody to bind to naphthalene was characterized by ELISA tests. A surface plasmon resonance (SPR) sensor platform was realized by sputtering a gold layer on a modified plastic optical fiber (POF). The gold surface was derivatizated and functionalized with the produced antibody by using the EDC/NHS amino-coupling immobilization protocol. The obtained results indicated that the POF-biosensor is able to sense the presence of naphthalene in a sea-water solution. The limit of detection (LOD) value was calculated to be 0.76 ng/mL, a value lower than the maximum residue limit value of naphthalene (0.13 µg/mL) referred as the water environmental quality standards (EQS). In addition, to the high sensitivity of the assay, it is remarkable to point out the possibility to monitor the presence of naphthalene in a real sea water solution by exploiting a simple experimental setup with a remote sensing capability offered by the POF-biosensor.
- Subjects :
- Optical fiber
Maximum Residue Limit
Analytical chemistry
02 engineering and technology
Naphthalenes
01 natural sciences
Biochemistry
law.invention
Analytical Chemistry
chemistry.chemical_compound
law
Limit of Detection
Surface plasmon resonance
Seawater
Plastic optical fiber
Plasmon
Optical Fibers
Spectroscopy
Naphthalene
Detection limit
Optical fiber sensor
Chemistry
Optical fiber sensors
010401 analytical chemistry
Chemistry (all)
021001 nanoscience & nanotechnology
0104 chemical sciences
Biosensors
13. Climate action
0210 nano-technology
Biosensor
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Talanta (Oxf.) 194 (2019): 289–297. doi:10.1016/j.talanta.2018.10.051, info:cnr-pdr/source/autori:Cennamo, Nunzio; Zeni, Luigi; Ricca, Ezio; Isticato, Rachele; Marzullo, Vincenzo Manuel; Capo, Alessandro; Staiano, Maria; D'Auria, Sabato; Varriale, Antonio/titolo:Detection of naphthalene in sea-water by a label-free plasmonic optical fiber biosensor/doi:10.1016%2Fj.talanta.2018.10.051/rivista:Talanta (Oxf.)/anno:2019/pagina_da:289/pagina_a:297/intervallo_pagine:289–297/volume:194
- Accession number :
- edsair.doi.dedup.....ce236eba759433ec97d9de6627a58f04