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Ambient mass spectrometry for the intraoperative molecular diagnosis of human brain tumors
- Source :
- Proceedings of the National Academy of Sciences. 110:1611-1616
- Publication Year :
- 2013
- Publisher :
- Proceedings of the National Academy of Sciences, 2013.
-
Abstract
- The main goal of brain tumor surgery is to maximize tumor resection while preserving brain function. However, existing imaging and surgical techniques do not offer the molecular information needed to delineate tumor boundaries. We have developed a system to rapidly analyze and classify brain tumors based on lipid information acquired by desorption electrospray ionization mass spectrometry (DESI-MS). In this study, a classifier was built to discriminate gliomas and meningiomas based on 36 glioma and 19 meningioma samples. The classifier was tested and results were validated for intraoperative use by analyzing and diagnosing tissue sections from 32 surgical specimens obtained from five research subjects who underwent brain tumor resection. The samples analyzed included oligodendroglioma, astrocytoma, and meningioma tumors of different histological grades and tumor cell concentrations. The molecular diagnosis derived from mass-spectrometry imaging corresponded to histopathology diagnosis with very few exceptions. Our work demonstrates that DESI-MS technology has the potential to identify the histology type of brain tumors. It provides information on glioma grade and, most importantly, may help define tumor margins by measuring the tumor cell concentration in a specimen. Results for stereotactically registered samples were correlated to preoperative MRI through neuronavigation, and visualized over segmented 3D MRI tumor volume reconstruction. Our findings demonstrate the potential of ambient mass spectrometry to guide brain tumor surgery by providing rapid diagnosis, and tumor margin assessment in near–real time.
- Subjects :
- Spectrometry, Mass, Electrospray Ionization
Pathology
medicine.medical_specialty
Neuronavigation
Oligodendroglioma
Plasmalogens
Phosphatidylserines
Astrocytoma
Phosphatidylinositols
Sensitivity and Specificity
Diagnosis, Differential
Stereotaxic Techniques
Meningioma
Imaging, Three-Dimensional
Monitoring, Intraoperative
Glioma
Meningeal Neoplasms
medicine
Humans
Multidisciplinary
medicine.diagnostic_test
Brain Neoplasms
business.industry
Reproducibility of Results
Magnetic resonance imaging
medicine.disease
Magnetic Resonance Imaging
Physical Sciences
Stereotaxic technique
Histopathology
business
Subjects
Details
- ISSN :
- 10916490 and 00278424
- Volume :
- 110
- Database :
- OpenAIRE
- Journal :
- Proceedings of the National Academy of Sciences
- Accession number :
- edsair.doi.dedup.....ce19b5a43e5fe4d5dbead515fe1e3fcd