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In vitro and in vivo pro-angiogenic effects of thymosin-beta 4-derived peptides

Authors :
Monica Dettin
Maria Teresa Conconi
Gabriella D'Auria
Carlo Di Bello
Diego Guidolin
Beatrice Nico
Lucia Falcigno
Francesca Ghezzo
Pier Paolo Parnigotto
Luca Urbani
Domenico Ribatti
Dettin, M.
Ghezzo, F.
Conconi, M. T.
Urbani, L.
D'Auria, Gabriella
Falcigno, Lucia
Guidolin, D.
Nico, B.
Ribatti, D.
Di Bello, C.
Parnigotto, P. P.
Publication Year :
2011

Abstract

Thymosin-beta 4 (T beta 4) is a G-actin sequestering peptide involved in regeneration and remodeling of injured tissues. In this work, we have designed and synthesized three peptide sequences containing the N-terminus (TYB4-n), the central part (TYB4-i) or the C-terminus (TYB4-c) of T beta 4. All fragments are overlapping on the main central binding actin site. After a structural characterization, we have evaluated in vitro and in vivo their pro-angiogenic effects. The results of this study have shown that: (i) each fragment reproduces the native conformation; (ii) T beta 4-derived peptides exert both in vitro and in vivo pro-angiogenic effects; (iii) their in vitro effect seem to be related to the activation of several signaling pathways and is positively modulated by the N-terminus of T beta 4. (C) 2011 Elsevier Inc. All rights reserved.

Details

Language :
English
Database :
OpenAIRE
Accession number :
edsair.doi.dedup.....ce03c75b691e5169e8885bb25fecb856