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In vitro and in vivo pro-angiogenic effects of thymosin-beta 4-derived peptides
- Publication Year :
- 2011
-
Abstract
- Thymosin-beta 4 (T beta 4) is a G-actin sequestering peptide involved in regeneration and remodeling of injured tissues. In this work, we have designed and synthesized three peptide sequences containing the N-terminus (TYB4-n), the central part (TYB4-i) or the C-terminus (TYB4-c) of T beta 4. All fragments are overlapping on the main central binding actin site. After a structural characterization, we have evaluated in vitro and in vivo their pro-angiogenic effects. The results of this study have shown that: (i) each fragment reproduces the native conformation; (ii) T beta 4-derived peptides exert both in vitro and in vivo pro-angiogenic effects; (iii) their in vitro effect seem to be related to the activation of several signaling pathways and is positively modulated by the N-terminus of T beta 4. (C) 2011 Elsevier Inc. All rights reserved.
- Subjects :
- Models, Molecular
Protein Conformation
Molecular Sequence Data
Immunology
Neovascularization, Physiologic
Peptide
Conformational studie
Chick Embryo
In Vitro Techniques
Biology
Protein structure
In vivo
Human Umbilical Vein Endothelial Cells
Animals
Humans
Amino Acid Sequence
Peptide sequence
Actin
Cell Proliferation
chemistry.chemical_classification
Wound Healing
Regeneration (biology)
Peptide Fragments
In vitro
NMR
Thymosin
chemistry
Biochemistry
Angiogenesis Inducing Agents
Angiogenesis
Signal transduction
Signal Transduction
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....ce03c75b691e5169e8885bb25fecb856