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Screening of mitochondrial mutations in Saudi women diagnosed with gestational diabetes mellitus: A non-replicative case-control study
- Source :
- Saudi Journal of Biological Sciences, Saudi Journal of Biological Sciences, Vol 29, Iss 1, Pp 360-365 (2022)
- Publication Year :
- 2022
- Publisher :
- Elsevier BV, 2022.
-
Abstract
- Introduction Among metabolic disorders, gestational diabetes mellitus (GDM) is specified as hyperglycemia caused by glucose or carbohydrate intolerance defects. GDM is distinguished by oxidative stress, and has been connected to mitochondrial dysfunction. Previous studies have documented the relation between A12026G, A8344G and A3243G mutations in ND4, tRNALeu(UUR), and tRNALys genes in different modes of diabetes. Aim The purpose of this study was to investigate into the relationship between GDM women and common mitochondrial mutations including A12026, A8344G, and A3243G in Saudi women. Methods In this case-control study, we have opted 96 GDM and 102 non-GDM pregnant women and DNA was extracted using EDTA blood and based on specific primers, Polymerase Chain Reaction was followed and then Restriction Fragment Length Polymorphism (RFLP) analysis was performed. Restriction enzymes was cross-checked with Lambda DNA and 10% of the purified PCR products were performed the Sanger sequencing analysis to reconfirm the RFLP analysis of the studied results. Results None of the heterozygous and homozygous mutations were not observed in our study. All the subjects were turned to be homozygous normal genotypes. Conclusion This study confirms that A12026, A8344G, and A3243G mutations have no role in the Saudi women with GDM.
- Subjects :
- medicine.medical_specialty
endocrine system diseases
QH301-705.5
law.invention
symbols.namesake
law
Internal medicine
Diabetes mellitus
Genotype
medicine
Biology (General)
Gestational Diabetes
Polymerase chain reaction
Sanger sequencing
mtDNA
business.industry
Case-control study
nutritional and metabolic diseases
medicine.disease
Gestational diabetes
Restriction enzyme
Endocrinology
A12026G
symbols
Original Article
A3243G
A8344G
Restriction fragment length polymorphism
General Agricultural and Biological Sciences
business
Subjects
Details
- ISSN :
- 1319562X
- Volume :
- 29
- Database :
- OpenAIRE
- Journal :
- Saudi Journal of Biological Sciences
- Accession number :
- edsair.doi.dedup.....cdf673205f2f40e8a1cc893b9bcdeb47