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Symmetry breaking in hydrodynamic forces drives meiotic spindle rotation in mammalian oocytes
- Source :
- Science Advances
- Publication Year :
- 2020
- Publisher :
- American Association for the Advancement of Science (AAAS), 2020.
-
Abstract
- Actin cytoskeletal asymmetry produces unbalanced hydrodynamic forces to drive spindle rotation during mouse meiotic division.<br />Patterned cell divisions require a precisely oriented spindle that segregates chromosomes and determines the cytokinetic plane. In this study, we investigated how the meiotic spindle orients through an obligatory rotation during meiotic division in mouse oocytes. We show that spindle rotation occurs at the completion of chromosome segregation, whereby the separated chromosome clusters each define a cortical actomyosin domain that produces cytoplasmic streaming, resulting in hydrodynamic forces on the spindle. These forces are initially balanced but become unbalanced to drive spindle rotation. This force imbalance is associated with spontaneous symmetry breaking in the distribution of the Arp2/3 complex and myosin-II on the cortex, brought about by feedback loops comprising Ran guanosine triphosphatase signaling, Arp2/3 complex activity, and myosin-II contractility. The torque produced by the unbalanced hydrodynamic forces, coupled with a pivot point at the spindle midzone cortical contract, constitutes a unique mechanical system for meiotic spindle rotation.
- Subjects :
- Male
Cell division
Spindle Apparatus
Rotation
Models, Biological
Chromosomes
Chromosome segregation
Mice
03 medical and health sciences
0302 clinical medicine
Meiosis
Animals
Research Articles
030304 developmental biology
Anaphase
Myosin Type II
Physics
0303 health sciences
Multidisciplinary
urogenital system
Spindle midzone
SciAdv r-articles
Cell Biology
Spermatozoa
Actins
Cytoplasmic streaming
Ran
Hydrodynamics
Oocytes
Biophysics
Algorithms
Cell Division
030217 neurology & neurosurgery
Research Article
Subjects
Details
- ISSN :
- 23752548
- Volume :
- 6
- Database :
- OpenAIRE
- Journal :
- Science Advances
- Accession number :
- edsair.doi.dedup.....cdeb82ee2269f32cb019345ea81ba396