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Aneuploidy screening using circulating fetal cells in maternal blood by dual‐probe FISH protocol: a prospective feasibility study on a series of 172 pregnant women
- Source :
- Molecular Genetics & Genomic Medicine
- Publication Year :
- 2016
- Publisher :
- John Wiley and Sons Inc., 2016.
-
Abstract
- Background A long sought goal in medical genetics has been the replacement of invasive procedures for the detection of chromosomal aneuploidies by isolating and analyzing fetal cells or free fetal DNA from maternal blood, avoiding risk to the fetus. However, a rapid, simple, consistent, and low-cost procedure suitable for routine clinical practice has not yet been achieved. The purpose of this study was to assess the feasibility of predicting fetal aneuploidy by applying our recently established dual-probe FISH protocol to fetal cells isolated and enriched from maternal blood. Methods A total of 172 pregnant women underwent prospective testing for fetal aneuploidy by FISH analysis of fetal cells isolated from maternal blood. Results were compared with the karyotype determined through invasive procedures or at birth. Results Seven of the samples exhibited fetal aneuploidy, which was confirmed by invasive prenatal diagnosis procedures. After enrichment for fetal cells, the frequency of trisomic cells was at least double in samples from aneuploid pregnancies (range 0.38–0.90%) compared to samples from normal pregnancies (≤0.18%). One false negative result was also obtained. Conclusions Noninvasive prenatal aneuploidy screening using fetal cells isolated from maternal blood is feasible and could substantially reduce the need for invasive procedures.
- Subjects :
- 0301 basic medicine
medicine.medical_specialty
Genetic counseling
Aneuploidy
Prenatal diagnosis
circulating fetal cells
Biology
03 medical and health sciences
0302 clinical medicine
FISH
Genetics
medicine
Molecular Biology
Genetics (clinical)
Gynecology
Fetus
030219 obstetrics & reproductive medicine
genetic counseling
Obstetrics
Karyotype
Original Articles
medicine.disease
030104 developmental biology
Cell-free fetal DNA
Medical genetics
Fish
Original Article
NIPT
Subjects
Details
- Language :
- English
- ISSN :
- 23249269
- Volume :
- 4
- Issue :
- 6
- Database :
- OpenAIRE
- Journal :
- Molecular Genetics & Genomic Medicine
- Accession number :
- edsair.doi.dedup.....cde8ba7be92e4459ade0738b4beb7a54