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Epitope mapping of neutralizing monoclonal antibody in avian influenza A H5N1 virus hemagglutinin
- Source :
- Biochemical and biophysical research communications. 418(1)
- Publication Year :
- 2011
-
Abstract
- The global spread of highly pathogenic avian influenza A H5N1 viruses raises concerns about more widespread infection in the human population. Pre-pandemic vaccine for H5N1 clade 1 influenza viruses has been produced from the A/Viet Nam/1194/2004 strain (VN1194), but recent prevalent avian H5N1 viruses have been categorized into the clade 2 strains, which are antigenically distinct from the pre-pandemic vaccine. To understand the antigenicity of H5N1 hemagglutinin (HA), we produced a neutralizing monoclonal antibody (mAb12-1G6) using the pre-pandemic vaccine. Analysis with chimeric and point mutant HAs revealed that mAb12-1G6 bound to the loop (amino acid positions 140-145) corresponding to an antigenic site A in the H3 HA. mAb12-1G6 failed to bind to the mutant VN1194 HA when only 3 residues were substituted with the corresponding residues of the clade 2.1.3.2 A/Indonesia/5/05 strain (amino acid substitutions at positions Q142L, K144S, and S145P), suggesting that these amino acids are critical for binding of mAb12-1G6. Escape mutants of VN1194 selected with mAb12-1G6 carried a S145P mutation. Interestingly, mAb12-1G6 cross-neutralized clade 1 and clade 2.2.1 but not clade 2.1.3.2 or clade 2.3.4 of the H5N1 virus. We discuss the cross-reactivity, based on the amino acid sequence of the epitope.
- Subjects :
- Population
Molecular Sequence Data
Biophysics
Hemagglutinin (influenza)
Hemagglutinin Glycoproteins, Influenza Virus
Biology
medicine.disease_cause
Antibodies, Viral
Biochemistry
Epitope
Cell Line
Birds
Mice
Dogs
Influenza, Human
Influenza A virus
medicine
Animals
Humans
Amino Acid Sequence
Clade
education
Neutralizing antibody
Molecular Biology
education.field_of_study
Influenza A Virus, H5N1 Subtype
Immunodominant Epitopes
Antibodies, Monoclonal
Cell Biology
Virology
Antibodies, Neutralizing
Influenza A virus subtype H5N1
Epitope mapping
HEK293 Cells
Influenza in Birds
biology.protein
Epitope Mapping
Subjects
Details
- ISSN :
- 10902104
- Volume :
- 418
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Biochemical and biophysical research communications
- Accession number :
- edsair.doi.dedup.....cde8b244c91f35516bb8cbde2e32cce1