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αB-Crystallin in clear cell renal cell carcinoma: Tumor progression and prognostic significance
- Source :
- Urologic Oncology: Seminars and Original Investigations. 31:1367-1377
- Publication Year :
- 2013
- Publisher :
- Elsevier BV, 2013.
-
Abstract
- Objectives AlphaB-crystallin (αB-crystallin), a small heat shock protein, has been reported to be involved in the growth, antiapoptosis, migration, and chemoresistance of human malignancies. Materials and methods αB-crystallin expression in normal renal and clear cell renal cell carcinoma (ccRCC) tissues was examined with two-dimensional (2D) gel electrophoresis assays. Immunohistochemistry was conducted to determine the presence of αB-crystallin-positive tumor cells and staining intensity in 50 cases of ccRCC tissue samples. The association of αB-crystallin protein expression, clinicopathogic parameters and prognosis of ccRCC patients was also analyzed with Student's t-test and Kaplan-Meier analysis. Moreover, Western blot assays were performed to detect the protein expression of αB-crystallin in normal and tumor tissues and the alteration of cell cycle regulators in αB-crystallin-overexpressing cells. MTT (3-[4,5-dimethythiazol-2-yl]-2,5-diphenyltetrazolium bromide), BrdU, and transwell assays were performed to demonstrate the effects of αB-crystallin overexpression on cell growth, DNA synthesis and cell migration of ccRCC cells, respectively. Results The results showed the up-regulation of αB-crystallin expression in ccRCC tissues. Overall survival of ccRCC patients was significantly correlated with αB-crystallin expression in tumor tissues. We found that αB-crystallin overexpression increased the expression of cyclin A and the incorporation of BrdU, which may be related to the enhancement of cell growth. Transwell analyses demonstrated that presence of αB-crystallin overexpression enhanced cell migration in ccRCC cells. Furthermore, rapamycin-resistance of tumor cells was induced when αB-crystallin was overexpressed. Conclusions Our experimental findings highlight the importance of αB-crystallin in the tumor growth, migration, and target therapy-resistance of ccRCC cells.
- Subjects :
- Adult
Male
Proteomics
Cell Survival
Urology
Blotting, Western
Cyclin A
Kaplan-Meier Estimate
Western blot
Cell Movement
Cell Line, Tumor
medicine
Humans
Electrophoresis, Gel, Two-Dimensional
Carcinoma, Renal Cell
Aged
Cell Proliferation
Aged, 80 and over
Sirolimus
biology
medicine.diagnostic_test
Cell growth
alpha-Crystallin B Chain
Cell migration
Middle Aged
Cell cycle
Prognosis
medicine.disease
Immunohistochemistry
Molecular biology
Kidney Neoplasms
eye diseases
Clear cell renal cell carcinoma
Oncology
Drug Resistance, Neoplasm
Tumor progression
Multivariate Analysis
Disease Progression
biology.protein
Female
sense organs
Subjects
Details
- ISSN :
- 10781439
- Volume :
- 31
- Database :
- OpenAIRE
- Journal :
- Urologic Oncology: Seminars and Original Investigations
- Accession number :
- edsair.doi.dedup.....cdd1598ffa62f26b22cd5bc7044152f2