Back to Search
Start Over
Requirement for p56(lck) tyrosine kinase activation in Th subset differentiation
- Source :
- International Immunology. 10:577-591
- Publication Year :
- 1998
- Publisher :
- Oxford University Press (OUP), 1998.
-
Abstract
- The lymphocyte-specific protein tyrosine kinase p56(lck) (Lck) is well documented with regard to its role in regulating T cell activation and thymocyte development through delivery of signals via the mature alphabeta TCR as well as the pre-TCR. Little is known, however, about the role of Lck in Th cell subset differentiation in the periphery. Here, we assess the requirement for tyrosine kinase activation of Lck in Th1 and Th2 cell differentiation by using a dominant-negative Lck (DLGKR) transgenic (Tg) mice under the control of a lck distal promoter that directs high expression in mature T cells, in which splenic CD4 T cells developed normally. This Tg mouse provides a good experimental model system to investigate the roles of Lck in mature T cell function in vivo. We show that the catalytically inactive Lck protein at about twice-normal concentrations inhibits Th2 subset differentiation in vivo and in vitro, whilst leaving the maturation of the other T cell subset, Th1, intact. These data indicate a requirement for Lck activity in Th2 cell differentiation, and a differential dependence for Lck activity between Th2 and Th1 cell differentiation.
- Subjects :
- Heterozygote
Ovalbumin
Helper T lymphocyte
T cell
Cellular differentiation
Molecular Sequence Data
Immunology
Receptors, Antigen, T-Cell
Gene Expression
Mice, Transgenic
chemical and pharmacologic phenomena
Lymphocyte Activation
Mice
Th2 Cells
medicine
Animals
Immunology and Allergy
Amino Acid Sequence
Mice, Inbred BALB C
Tyrosine-protein kinase CSK
Chemistry
ZAP70
Antibodies, Monoclonal
CD28
Cell Differentiation
hemic and immune systems
General Medicine
Th1 Cells
Cell biology
Enzyme Activation
Mice, Inbred C57BL
Thymocyte
Phenotype
medicine.anatomical_structure
Lymphocyte Specific Protein Tyrosine Kinase p56(lck)
Antibody Formation
CD4 Antigens
Cancer research
Cytokines
biological phenomena, cell phenomena, and immunity
Tyrosine kinase
Subjects
Details
- ISSN :
- 14602377
- Volume :
- 10
- Database :
- OpenAIRE
- Journal :
- International Immunology
- Accession number :
- edsair.doi.dedup.....cdc99c9b3f3591f7a760afc997448916