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IL-34 is overexpressed in the inflamed salivary glands of patients with Sjogren's syndrome and is associated with the local expansion of pro-inflammatory CD14brightCD16+ monocytes
- Source :
- Rheumatology. 52:1009-1017
- Publication Year :
- 2013
- Publisher :
- Oxford University Press (OUP), 2013.
-
Abstract
- Objectives To investigate the expression of IL-34 in labial salivary glands (LSGs) of patients with primary SS (p-SS) and its role in inducing a pro-inflammatory monocyte phenotype. Methods LSG biopsies were obtained from 20 patients with p-SS and 10 patients with non-Sjogren's sicca syndrome (n-SS). The expression of IL-34, IL-1β, TNF-α, IL-17 and IL-23 was assessed by real-time PCR. IL-34 expression was also investigated in LSGs by immunohistochemistry. The frequencies of subpopulations of CD14(+) monocytes were evaluated by flow cytometry among isolated mononuclear cells from peripheral blood and salivary glands from both patients and controls. The role of recombinant IL-34 on isolated peripheral blood mononuclear cells was also evaluated. Results IL-34 m-RNA was overexpressed in the inflamed salivary glands of p-SS and associated with increased expression of TNF-α, IL-1β, IL-17 and IL-23p19. The increased expression of IL-34 was confirmed by immunohistochemistry in paraffin-embedded salivary glands from p-SS patients. IL-34 expression was accompanied by the expansion of pro-inflammatory CD14(bright)CD16(+) monocytes in the salivary glands. In vitro stimulation of peripheral blood mononuclear cells with IL-34 induced the expansion of both CD14(+)CD16(-) cells and CD14(bright)CD16(+) cells in p-SS and non-SS subjects. Conclusion IL-34 seems to be involved in the pathogenesis of salivary gland inflammation in p-SS.
- Subjects :
- Adult
Male
Pathology
medicine.medical_specialty
CD14
Interleukin-1beta
Lipopolysaccharide Receptors
Inflammation
CD16
Interleukin-23
Peripheral blood mononuclear cell
Monocytes
Salivary Glands
Rheumatology
Sicca syndrome
medicine
Humans
Pharmacology (medical)
interleukin-34, Sjögren’s syndrome, monocytes
Aged
Salivary gland
Tumor Necrosis Factor-alpha
business.industry
Interleukins
Monocyte
Interleukin-17
Receptors, IgG
Middle Aged
Flow Cytometry
Settore MED/16 - Reumatologia
Sjogren's Syndrome
medicine.anatomical_structure
Immunology
Interleukin 34
Female
medicine.symptom
business
Subjects
Details
- ISSN :
- 14620332 and 14620324
- Volume :
- 52
- Database :
- OpenAIRE
- Journal :
- Rheumatology
- Accession number :
- edsair.doi.dedup.....cdc24e96f41a13c781b90f85bf70abf7