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Delay expression of limonoid UDP-glucosyltransferase makes delayed bitterness in citrus

Authors :
Saman Hosseinkhani
Asad Asadi-Abkenar
Zabihollah Zamani
Reza Omidbaigi
Fariborz Zaare-Nahandi
Source :
Biochemical and Biophysical Research Communications. 371:59-62
Publication Year :
2008
Publisher :
Elsevier BV, 2008.

Abstract

Genes encoding limonoid UDP-glucosyltransferase from albedo of six Citrus species with different levels of delayed bitterness are isolated and cloned in vector pTZ57R/T. Our results indicate that gene sequence of sweet lime (with intense juice delayed bitterness) have complete identity with Satsuma mandarin (without distinctive juice delayed bitterness). Also gene sequence of Marsh seedless grapefruit, local orange and Thompson navel orange (with mild juice delayed bitterness) have very similarity with Satsuma mandarin. On the other hand, this gene started to express 60, 120, and 210 days after full blooming in albedo of Satsuma mandarin, sweet oranges and sour orange, and both grapefruit and sweet lime, respectively. Expression pattern of limonoid glucosyltransferase gene in leaves was quite different with albedo. Thus, we supposed the delayed bitterness in this species was related to delay in expression of limonoid glucosyltransferase gene in albedo and lower limonoid glucoside accumulation in fruits.

Details

ISSN :
0006291X
Volume :
371
Database :
OpenAIRE
Journal :
Biochemical and Biophysical Research Communications
Accession number :
edsair.doi.dedup.....cdb5e5f2ac6d30e60fd36161897d2c94