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Delay expression of limonoid UDP-glucosyltransferase makes delayed bitterness in citrus
- Source :
- Biochemical and Biophysical Research Communications. 371:59-62
- Publication Year :
- 2008
- Publisher :
- Elsevier BV, 2008.
-
Abstract
- Genes encoding limonoid UDP-glucosyltransferase from albedo of six Citrus species with different levels of delayed bitterness are isolated and cloned in vector pTZ57R/T. Our results indicate that gene sequence of sweet lime (with intense juice delayed bitterness) have complete identity with Satsuma mandarin (without distinctive juice delayed bitterness). Also gene sequence of Marsh seedless grapefruit, local orange and Thompson navel orange (with mild juice delayed bitterness) have very similarity with Satsuma mandarin. On the other hand, this gene started to express 60, 120, and 210 days after full blooming in albedo of Satsuma mandarin, sweet oranges and sour orange, and both grapefruit and sweet lime, respectively. Expression pattern of limonoid glucosyltransferase gene in leaves was quite different with albedo. Thus, we supposed the delayed bitterness in this species was related to delay in expression of limonoid glucosyltransferase gene in albedo and lower limonoid glucoside accumulation in fruits.
- Subjects :
- Citrus
Limonoid UDP-glucosyltransferase
Amino Acid Motifs
Molecular Sequence Data
Biophysics
Orange (colour)
Limonoid
Biochemistry
Beverages
chemistry.chemical_compound
stomatognathic system
Expression pattern
Glucoside
Gene Expression Regulation, Plant
Botany
Gene expression
medicine
Amino Acid Sequence
Molecular Biology
Phylogeny
Plant Proteins
biology
food and beverages
Sequence Analysis, DNA
Cell Biology
Protein Structure, Tertiary
Horticulture
chemistry
Glucosyltransferases
Taste
biology.protein
Glucosyltransferase
Navel orange
medicine.drug
Subjects
Details
- ISSN :
- 0006291X
- Volume :
- 371
- Database :
- OpenAIRE
- Journal :
- Biochemical and Biophysical Research Communications
- Accession number :
- edsair.doi.dedup.....cdb5e5f2ac6d30e60fd36161897d2c94