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Enhanced arbovirus surveillance with deep sequencing: Identification of novel rhabdoviruses and bunyaviruses in Australian mosquitoes
- Source :
- Virology. :146-158
- Publisher :
- Elsevier Inc.
-
Abstract
- Viral metagenomics characterizes known and identifies unknown viruses based on sequence similarities to any previously sequenced viral genomes. A metagenomics approach was used to identify virus sequences in Australian mosquitoes causing cytopathic effects in inoculated mammalian cell cultures. Sequence comparisons revealed strains of Liao Ning virus (Reovirus, Seadornavirus), previously detected only in China, livestock-infecting Stretch Lagoon virus (Reovirus, Orbivirus), two novel dimarhabdoviruses, named Beaumont and North Creek viruses, and two novel orthobunyaviruses, named Murrumbidgee and Salt Ash viruses. The novel virus proteomes diverged by ≥50% relative to their closest previously genetically characterized viral relatives. Deep sequencing also generated genomes of Warrego and Wallal viruses, orbiviruses linked to kangaroo blindness, whose genomes had not been fully characterized. This study highlights viral metagenomics in concert with traditional arbovirus surveillance to characterize known and new arboviruses in field-collected mosquitoes. Follow-up epidemiological studies are required to determine whether the novel viruses infect humans.
- Subjects :
- Viral metagenomics
Orthobunyavirus
Deep sequencing
viruses
Molecular Sequence Data
Genome, Viral
Reovirus
Bunyaviridae Infections
Arbovirus
Article
Liao ning virus
Mosquito
Kunjin virus
Virology
Banna virus
Rhabdoviridae Infections
medicine
Animals
Humans
Novel virus
Virus discovery
Phylogeny
Genetics
Orbivirus
biology
Arbovirus surveillance
Epizootic hemorrhagic disease virus
Australia
High-Throughput Nucleotide Sequencing
Rhabdovirus
biology.organism_classification
medicine.disease
3. Good health
Insect Vectors
Culicidae
Bunyavirus
Rhabdoviridae
Sentinel Surveillance
Subjects
Details
- Language :
- English
- ISSN :
- 00426822
- Database :
- OpenAIRE
- Journal :
- Virology
- Accession number :
- edsair.doi.dedup.....cdb445732dc15194daedca33a7db659d
- Full Text :
- https://doi.org/10.1016/j.virol.2013.09.026