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A helicase-containing module defines a family of pCD630-like plasmids in Clostridium difficile
- Source :
- Anaerobe, 49, 78-84
- Publication Year :
- 2018
-
Abstract
- Clostridium difficile is a Gram-positive and sporulating enteropathogen that is a major cause of healthcare-associated infections. Even though a large number of genomes of this species have been sequenced, only a few plasmids have been described in the literature. Here, we use a combination of in silico analyses and laboratory experiments to show that plasmids are common in C. difficile. We focus on a group of plasmids that share similarity with the plasmid pCD630, from the reference strain 630. The family of pCD630-like plasmids is defined by the presence of a conserved putative helicase that is likely part of the plasmid replicon. This replicon is compatible with at least some other C. difficile replicons, as strains can carry pCD630-like plasmids in addition to other plasmids. We find two distinct sub-groups of pCD630-like plasmids that differ in size and accessory modules. This study is the first to describe a family of plasmids in C. difficile.
- Subjects :
- 0301 basic medicine
qu_137
In silico
Replication
wc_368
Microbiology
Genome
Plasmid
Helicase
03 medical and health sciences
Bacterial Proteins
Humans
Replicon
030304 developmental biology
Genetics
0303 health sciences
Strain (chemistry)
biology
Clostridioides difficile
030306 microbiology
Strain (biology)
DNA Helicases
Clostridium difficile
Virology
qw_51
030104 developmental biology
Infectious Diseases
Clostridium Infections
biology.protein
qu_470
qw_142
Plasmids
Subjects
Details
- Language :
- English
- ISSN :
- 10759964
- Database :
- OpenAIRE
- Journal :
- Anaerobe, 49, 78-84
- Accession number :
- edsair.doi.dedup.....cdb300d7233a4d790445b2e3a88192cb