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Sequence analysis suggests a recent duplication of the growth hormoneencoding gene in Tilapia nilotica
- Source :
- Gene. 125:143-150
- Publication Year :
- 1993
- Publisher :
- Elsevier BV, 1993.
-
Abstract
- The sequence of two growth hormone(GH)-encoding genes from tilapia fish (Tilapia nilotica) is reported. Our data indicate that the presence of two GH in the tilapia genome is a consequence of a relatively recent duplication event. The two genes are highly homologous, having a similar intron (five)/exon (six) arrangement, and both encode an identical polypeptide. Sequence similarity extends up to bp -628 upstream to the transcription start point, after which the sequences of the two genes are not related to each other. The presence of two GH in the tilapia genome is supported both by the nucleotide sequence and by genomic DNA blot hybridization analysis. Tilapias, like salmonids, contain an extra intron compared with the mammalian GH structure. We suggest that within the superorder Teleostei, the insertion of intron 5 into GH took place after the evolutionary separation of Cyprinoidea, but before Isospondyli (salmonids) and Acanthopterygii (tilapias) were separated. Thus, the additional intron which is probably present in many teleost fish GH may provide an excellent natural marker for evolution and classification studies.
- Subjects :
- Genetics
Base Sequence
Sequence analysis
Molecular Sequence Data
Restriction Mapping
Nucleic acid sequence
Intron
DNA
Sequence Analysis, DNA
General Medicine
Biology
Genome
Conserved sequence
Blotting, Southern
Molecular evolution
Growth Hormone
Multigene Family
Gene duplication
Animals
Amino Acid Sequence
human activities
Gene
Conserved Sequence
Tilapia
Subjects
Details
- ISSN :
- 03781119
- Volume :
- 125
- Database :
- OpenAIRE
- Journal :
- Gene
- Accession number :
- edsair.doi.dedup.....cdadc3faad2925f8d6c81f6083299f3c