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Feasibility and safety study of 22-gauge endoscopic ultrasound (EUS) needles for portal vein sampling in a swine model
- Source :
- Endoscopy International Open, Vol 08, Iss 11, Pp E1717-E1724 (2020), Endoscopy International Open
- Publication Year :
- 2020
- Publisher :
- Georg Thieme Verlag KG, 2020.
-
Abstract
- Background and study aims Endoscopic ultrasound (EUS) has been used for portal vein sampling in patients with pancreaticobiliary cancers for enumerating circulating tumor cells but is not yet a standard procedure. Further evaluation is needed to refine the methodology. Therefore, we evaluated the feasibility and safety of 19-gauge (19G) versus a 22-gauge (22 G) EUS fine-needle aspiration needles for portal vein sampling in a swine model. Methods Celiotomy was performed on two farm pigs. Portal vein sampling occurred transhepatically. We compared 19 G and 22 G needles coated interiorly with saline, heparin or ethylenediaminetetraacetic acid (EDTA). Small- (10 mL) and large- (25 mL) volume blood collections were evaluated. Two different collection methods were tested: direct-to-vial and suction syringe. A bleeding risk trial for saline-coated 19 G and 22 G needles was performed by puncturing the portal vein 20 times. Persistent bleeding after 3 minutes was considered significant. Results All small-volume collection trials were successful except for 22 G saline-coated needles with direct-to-vial method. All large-volume collection trials were successful when using suction syringe; direct-to-vial method for both 19 G and 22 G needles were unsuccessful. Collection times were shorter for 19 G vs. 22 G needles for both small and large-volume collections (P Conclusion The results of this animal study demonstrate the feasibility and the safety of using 22 G needles for portal vein sampling and can form the basis for a pilot study in patients.
- Subjects :
- Suction (medicine)
Endoscopic ultrasound
medicine.medical_specialty
Original article
medicine.diagnostic_test
business.industry
medicine.medical_treatment
Ultrasound
Portal vein
03 medical and health sciences
0302 clinical medicine
030220 oncology & carcinogenesis
medicine
030211 gastroenterology & hepatology
Pharmacology (medical)
Sampling (medicine)
lcsh:Diseases of the digestive system. Gastroenterology
Radiology
lcsh:RC799-869
business
Saline
Fine-needle aspirate
Syringe
Subjects
Details
- Language :
- English
- ISSN :
- 21969736 and 23643722
- Issue :
- 11
- Database :
- OpenAIRE
- Journal :
- Endoscopy International Open
- Accession number :
- edsair.doi.dedup.....cd8b2b03f6503246faef5fb84d8fcc51