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Co-cultures of multiple cell types mimic pulmonary cell communication in response to urban PM10
- Source :
- The European respiratory journal. 32(5)
- Publication Year :
- 2008
-
Abstract
- The current authors evaluated whether a system of co-cultures of relevant cells (pneumocytes (A549), macrophages (THP-1), mast cells (HMC-1) and endothelial cells (EAHY926)) would mimic the responses to particles with a 50% cut-off aerodynamic diameter of 10 microm (PM(10)) previously reported in vivo. The role of mast cells was considered of special interest. Single cultures, bicultures (A549 + HMC-1 in a 10:1 ratio; THP-1 + HMC-1 in a 2:1 ratio) and tricultures (A549 + THP-1 + HMC-1 in a 10:2:1 ratio) were exposed to urban PM(10) (24 h at 0, 10, 30 or 100 microg x cm(-2)). Additionally, EAHY926 cells were introduced in inserts above the tricultures. The released cytokines were evaluated with a fluorescence-activated cell sorter array system. THP-1 + HMC-1 bicultures and the tricultures released more granulocyte colony-stimulating factor (G-CSF), macrophage inflammatory protein (MIP)-1beta, interleukin (IL)-1beta, IL-8, IL-6, tumour necrosis factor-alpha and MIP-1alpha in response to PM(10) than the sum of the single cultures. Tricultures with EAHY926 released more G-CSF, MIP-1alpha, IL-8 and MIP-1beta than the EAHY926 single culture. The bicultures, tricultures and tricultures with EAHY926 provide results that are consistent with the local and systemic effects previously described for particulate matter effects, i.e. inflammation, endothelial dysfunction and bone marrow cell mobilisation. Mast cells seem to play a significant role in the co-culture responses.
- Subjects :
- Pulmonary and Respiratory Medicine
Cell type
Necrosis
medicine.medical_treatment
Interleukin-1beta
Protein Array Analysis
Inflammation
Granulocyte
Models, Biological
Cell Line, Tumor
Granulocyte Colony-Stimulating Factor
medicine
Humans
Mast Cells
Particle Size
Chemokine CCL4
Macrophage inflammatory protein
business.industry
Interleukin-6
Tumor Necrosis Factor-alpha
Macrophages
Interleukin-8
Interleukin
Endothelial Cells
Molecular biology
Coculture Techniques
medicine.anatomical_structure
Cytokine
Cell culture
Immunology
medicine.symptom
business
Subjects
Details
- ISSN :
- 13993003
- Volume :
- 32
- Issue :
- 5
- Database :
- OpenAIRE
- Journal :
- The European respiratory journal
- Accession number :
- edsair.doi.dedup.....cd5bbce5ac7440cd11538a037605655e