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RhoA/rock signaling mediates peroxynitrite-induced functional impairment of Rat coronary vessels
- Source :
- BMC Cardiovascular Disorders
- Publication Year :
- 2016
- Publisher :
- BioMed Central, 2016.
-
Abstract
- Background Diabetes-induced vascular dysfunction may arise from reduced nitric oxide (NO) availability, following interaction with superoxide to form peroxynitrite. Peroxynitrite can induce formation of 3-nitrotyrosine-modified proteins. RhoA/ROCK signaling is also involved in diabetes-induced vascular dysfunction. The study aimed to investigate possible links between Rho/ROCK signaling, hyperglycemia, and peroxynitrite in small coronary arteries. Methods Rat small coronary arteries were exposed to normal (NG; 5.5 mM) or high (HG; 23 mM) D-glucose. Vascular ring constriction to 3 mM 4-aminopyridine and dilation to 1 μM forskolin were measured. Protein expression (immunohistochemistry and western blot), mRNA expression (real-time PCR), and protein activity (luminescence-based G-LISA and kinase activity spectroscopy assays) of RhoA, ROCK1, and ROCK2 were determined. Results Vascular ring constriction and dilation were smaller in the HG group than in the NG group (P
- Subjects :
- 0301 basic medicine
Male
RHOA
Vasodilation
Coronary Artery Disease
030204 cardiovascular system & hematology
Peroxynitrite
Rats, Sprague-Dawley
chemistry.chemical_compound
0302 clinical medicine
ROCK1
Phosphorylation
Cells, Cultured
rho-Associated Kinases
biology
Coronary Vessels
Immunohistochemistry
Cardiology
medicine.symptom
Cardiology and Cardiovascular Medicine
Research Article
Signal Transduction
medicine.medical_specialty
Blotting, Western
Coronary artery
Real-Time Polymerase Chain Reaction
Nitric oxide
Diabetes Mellitus, Experimental
03 medical and health sciences
Internal medicine
Peroxynitrous Acid
medicine
Animals
Kinase activity
business.industry
Rats
Peroxynitrous acid
Disease Models, Animal
030104 developmental biology
Endocrinology
chemistry
Gene Expression Regulation
Vasoconstriction
Hyperglycemia
biology.protein
RhoA/ROCK
RNA
business
rhoA GTP-Binding Protein
Subjects
Details
- Language :
- English
- ISSN :
- 14712261
- Volume :
- 16
- Database :
- OpenAIRE
- Journal :
- BMC Cardiovascular Disorders
- Accession number :
- edsair.doi.dedup.....cd2f5ff38f2d666f7b5098d4d3ac329c