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Ischemic Preconditioning and Intermittent Clamping Increase the Tolerance of Fatty Liver to Hepatic Ischemia-Reperfusion Injury in the Rat
- Source :
- Transplantation Proceedings. 39:3010-3014
- Publication Year :
- 2007
- Publisher :
- Elsevier BV, 2007.
-
Abstract
- Liver ischemia-reperfusion (I/R) injury is a well-known cause of morbidity and mortality following liver surgery and transplantation. Hepatic steatosis increases the extent of cellular injury incurred during I/R injury. We sought to identify measures that reduced the untoward sequelae of liver I/R injury.Male Zucker rats were subjected to 75 minutes of 70% hepatic ischemia, and 3 hours of reperfusion. The ischemic periods were based on the following protocols: continuous clamping (CC) for 75 minutes; intermittent clamping (IC) with five cycles of 15 minutes clamp on and 5 minutes clamp off; or ischemic preconditioning (IP) with 10 minutes clamp on, 15 minutes off, and 60 minutes on (n=7 in each group). Warm I/R injury was evaluated using serum levels of aspartate aminotransferase (AST), serum interleukin (IL)-6, as well as hematoxylin and eosin staining.Hepatocellular injury was significantly reduced with IP or IC compared with CC (AST: 3285+/-122.3 and 2875+/-285.4 compared with 5436.3+/--984.7 units/L, respectively; P.01). Serum IL-6 level was also significantly reduced with IP and IC compared with CC (70+/-8.8 and 76+/-6.2 compared with 147+/-8.5 ng/l, respectively (p.01). Histological analysis also revealed that IC and IP provided significant protection compared with the CC group.IC and IP increased the tolerance of a fatty liver to hepatic I/R injury.
- Subjects :
- Male
Pathology
medicine.medical_specialty
Ischemia
H&E stain
Anesthesia, General
medicine
Animals
Ischemic Preconditioning
Transplantation
business.industry
Fatty liver
medicine.disease
Rats
Rats, Zucker
Fatty Liver
Clamp
Liver
Reperfusion Injury
Anesthesia
Ischemic preconditioning
Surgery
Steatosis
business
Reperfusion injury
Liver Circulation
Subjects
Details
- ISSN :
- 00411345
- Volume :
- 39
- Database :
- OpenAIRE
- Journal :
- Transplantation Proceedings
- Accession number :
- edsair.doi.dedup.....cd1b744318a44c36b31726c8a8cf926f