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Feasibility of next-generation sequencing in clinical practice: results of a pilot study in the Department of Precision Medicine at the University of Campania ‘Luigi Vanvitelli’
- Source :
- ESMO Open
- Publication Year :
- 2020
- Publisher :
- BMJ Publishing Group, 2020.
-
Abstract
- Background The emerging role of next-generation sequencing (NGS) targeted panels is revolutionising our approach to cancer patients, providing information on gene alterations helpful for diagnosis and clinical decision, in a short time and with acceptable costs. Materials and methods In this work, we evaluated the clinical application of FoundationOne CDx test, a hybrid capture-based NGS. This test identifies alterations in 324 genes, tumour mutational burden and genomic signatures as microsatellite instability. The decision to obtain the NGS assay for a particular patient was done according to investigator's choice. Results Overall, 122 tumour specimens were analysed, of which 84 (68.85%) succeeded. The success rate was influenced by type of specimen formalin-fixed paraffin embedded (FFPE block vs FFPE slides), by origin of the sample (surgery vs biopsy) and by time of fixation (
- Subjects :
- Oncology
Cancer Research
medicine.medical_specialty
foundationone
Colorectal cancer
Population
medicine.disease_cause
Breast cancer
Internal medicine
medicine
Lung cancer
education
Original Research
education.field_of_study
Performance status
business.industry
comprehensive genomic profiling
CGP
Cancer
medicine.disease
NGS
next-generation sequencing
KRAS
Ovarian cancer
business
Subjects
Details
- Language :
- English
- ISSN :
- 20597029
- Volume :
- 5
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- ESMO Open
- Accession number :
- edsair.doi.dedup.....cd171d7e7cf792dacc788cb751f721d4