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Ribosome display and selection of singleāchain variable fragments effectively inhibit growth and progression of microspheres in vitro and in vivo
- Source :
- Cancer Science
- Publication Year :
- 2018
- Publisher :
- Wiley, 2018.
-
Abstract
- Distinguishing the surface markers of cancer stem cells (CSCs) is a useful method for early diagnosis and treatment of tumors, as CSCs may participate in tumorigenesis and metastasis by migrating into the circulatory system. However, the potential targets of CSCs are expressed at low levels in the natural state and are always changing. Thus, dynamic screening has been reported to be an effective measure for exploring CSC markers. In recent years, diverse single-chain variable fragments (scFvs) have been widely used in immunotherapy. In this study, we determined that the scFvs, screened using RD, had a high affinity to microspheres and could inhibit their progression. We also observed that the selected scFvs underwent evolution in vitro, and antitumor-associated proteins were successfully expressed. Combined with chemotherapy, the scFvs had a synergistic effect on the inhibition of the microspheres' progression in vitro and in vivo, which could be ascribed to their high affinity for stem-like cells and the inhibition of the microspheres' collective behaviors. In addition, proteins inhibiting CD44+ /CD24+ and MAPK were involved. Our data indicated that dynamic screening of the scFvs in a natural state was of great significance in the inhibition of the microspheres in vitro and in vivo.
- Subjects :
- 0301 basic medicine
Cancer Research
medicine.medical_treatment
Breast Neoplasms
medicine.disease_cause
scFv
Mice
03 medical and health sciences
0302 clinical medicine
Drug Therapy
Cell, Molecular, and Stem Cell Biology
Cancer stem cell
In vivo
Cell Line, Tumor
medicine
Animals
Humans
Cell Proliferation
biology
ribosome display
Chemistry
Cell growth
screening
CD44
Drug Synergism
Original Articles
General Medicine
Immunotherapy
Xenograft Model Antitumor Assays
Microspheres
In vitro
Cell biology
Gene Expression Regulation, Neoplastic
030104 developmental biology
Oncology
030220 oncology & carcinogenesis
microsphere
Ribosome display
Neoplastic Stem Cells
biology.protein
Female
Original Article
Cell Surface Display Techniques
CD44+/CD24+
Carcinogenesis
Ribosomes
Single-Chain Antibodies
Subjects
Details
- ISSN :
- 13497006 and 13479032
- Volume :
- 109
- Database :
- OpenAIRE
- Journal :
- Cancer Science
- Accession number :
- edsair.doi.dedup.....ccd5a9909f8ec3e02c3141bdff1e9afa