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SKP1-like protein, CrSKP1-e, interacts with pollen-specific F-box proteins and assembles into SCF-type E3 complex in ‘Wuzishatangju’ (Citrus reticulata Blanco) pollen
- Source :
- PeerJ
- Publication Year :
- 2020
- Publisher :
- PeerJ Inc., 2020.
-
Abstract
- S-ribonuclease (S-RNase)-based self-incompatibility (SI) mechanisms have been extensively studied in Solanaceae, Rosaceae and Plantaginaceae. S-RNase-based SI is controlled by two closely related genes, S-RNase and S-locus F-box (SLF), located at a polymorphic S-locus. In the SI system, the SCF-type (SKP1-CUL1-F-box-RBX1) complex functions as an E3 ubiquitin ligase complex for ubiquitination of non-self S-RNase. Pummelo (Citrus grandis) and several mandarin cultivars are suggested to utilize an S-RNase-based SI system. However, the molecular mechanism of the non-S-factors involved in the SI reaction is not straightforward in Citrus. To investigate the SCF-type E3 complex responsible for the SI reaction in mandarin, SLF, SKP1-like and CUL1 candidates potentially involved in the SI reaction of ‘Wuzishatangju’ (Citrus reticulata Blanco) were identified based on the genome-wide identification and expression analyses. Sixteen pollen-specific F-box genes (CrFBX1-CrFBX16), one pollen-specific SKP1-like gene (CrSKP1-e) and two CUL1 genes (CrCUL1A and CrCUL1B) were identified and cloned from ‘Wuzishatangju’. Yeast two-hybrid (Y2H) and in vitro binding assays showed that five CrFBX proteins could bind to CrSKP1-e, which is an ortholog of SSK1 (SLF-interacting-SKP1-like), a non-S-factor responsible for the SI reaction. Luciferase complementation imaging (LCI) and in vitro binding assays also showed that CrSKP1-e interacts with the N-terminal region of both CrCUL1A and CrCUL1B. These results indicate that CrSKP1-e may serve as a functional member of the SCF-type E3 ubiquitin ligase complex in ‘Wuzishatangju’.
- Subjects :
- 0106 biological sciences
SCF complex
F-box
Plant Science
01 natural sciences
F-box protein
General Biochemistry, Genetics and Molecular Biology
Self-incompatibility
03 medical and health sciences
Skp1
Luciferase
SKP1-like
Agricultural Science
Gene
Molecular Biology
Citrus retuculata Blanco
030304 developmental biology
0303 health sciences
biology
Chemistry
General Neuroscience
General Medicine
Cullin1
Ubiquitin ligase
Complementation
Biochemistry
biology.protein
CUL1
General Agricultural and Biological Sciences
010606 plant biology & botany
Biotechnology
Subjects
Details
- Language :
- English
- ISSN :
- 21678359
- Volume :
- 8
- Database :
- OpenAIRE
- Journal :
- PeerJ
- Accession number :
- edsair.doi.dedup.....cccd374de72ba4c933c19e4681c921db