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Characterization of Rhamnolipids Produced by an Arctic Marine Bacterium from the Pseudomonas fluorescence Group
- Source :
- Marine drugs, vol 16, iss 5, Marine Drugs, Marine Drugs; Volume 16; Issue 5; Pages: 163
- Publication Year :
- 2018
- Publisher :
- MDPI, 2018.
-
Abstract
- The marine environment is a rich source of biodiversity, including microorganisms that have proven to be prolific producers of bioactive secondary metabolites. Arctic seas are less explored than warmer, more accessible areas, providing a promising starting point to search for novel bioactive compounds. In the present work, an Arctic marine Pseudomonas sp. belonging to the Pseudomonas (P.) fluorescence group was cultivated in four different media in an attempt to activate biosynthetic pathways leading to the production of antibacterial and anticancer compounds. Culture extracts were pre-fractionated and screened for antibacterial and anticancer activities. One fraction from three of the four growth conditions showed inhibitory activity towards bacteria and cancer cells. The active fractions were dereplicated using molecular networking based on MS/MS fragmentation data, indicating the presence of a cluster of related rhamnolipids. Six compounds were isolated using HPLC and mass-guided fractionation, and by interpreting data from NMR and high-resolution MS/MS analysis; the structures of the compounds were determined to be five mono-rhamnolipids and the lipid moiety of one of the rhamnolipids. Molecular networking proved to be a valuable tool for dereplication of these related compounds, and for the first time, five mono-rhamnolipids from a bacterium within the P. fluorescence group were characterized, including one new mono-rhamnolipid.
- Subjects :
- 0301 basic medicine
Aquatic Organisms
many compounds)
arctic bacteria
Microorganism
Pharmaceutical Science
Chemical Fractionation
Drug Screening Assays
Rhamnose
bioactive
OSMAC (one strain, many compounds)
molecular networking
rhamnolipids
Drug Discovery
Pharmacology, Toxicology and Pharmaceutics (miscellaneous)
biology
Arctic Regions
Chemistry
Rhamnolipids
Pseudomonas
Pharmacology and Pharmaceutical Sciences
Fluorescence
Anti-Bacterial Agents
Biochemistry
Molecular networking
Physical Chemistry (incl. Structural)
Medicinal & Biomolecular Chemistry
Antineoplastic Agents
Microbial Sensitivity Tests
Fractionation
OSMAC
Article
03 medical and health sciences
VDP::Teknologi: 500::Bioteknologi: 590
VDP::Technology: 500::Biotechnology: 590
Arctic bacteria
14. Life underwater
Life Below Water
Decanoates
Ms analysis
Antitumor
biology.organism_classification
Biosynthetic Pathways
030104 developmental biology
Arctic
Bioactive
OSMAC (one strain
Drug Screening Assays, Antitumor
Bacteria
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Marine drugs, vol 16, iss 5, Marine Drugs, Marine Drugs; Volume 16; Issue 5; Pages: 163
- Accession number :
- edsair.doi.dedup.....ccc9b915d7c82b87205049e4f696ea7e