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Differential autophagic effects of vital dyes in retinal pigment epithelial ARPE-19 and photoreceptor 661W cells
- Source :
- PLoS ONE, Vol 12, Iss 3, p e0174736 (2017), PLoS ONE
- Publication Year :
- 2017
- Publisher :
- Public Library of Science (PLoS), 2017.
-
Abstract
- Indocyanine green (ICG) and brilliant blue G (BBG) are commonly used vital dyes to remove internal limiting membrane (ILM) in vitreoretinal surgery. The vital dyes have shown cytotoxic effects in ocular cells. Autophagy is a stress responsive pathway for either protecting cells or promoting cell death. However, the role of autophagy in ocular cells in response to the vital dyes remains unknown. In this study, we found that ICG and BBG reduced cell viability in both human retinal pigment epithelial ARPE-19 and mouse photoreceptor 661W cells. ICG and BBG induced lipidated GFP-LC3-II and LC3-II in ARPE-19 and 661W cells. Combination treatment with the autophagy inhibitor chloroquine indicated that ICG and BBG reduced autophagic flux in ARPE-19 cells, whereas the vital dyes induced autophagic flux in 661W cells. Moreover, genetic and pharmacological ablation of autophagy enhanced vital dyes-induced cytotoxicity in ocular cells. Dietary supplements, including resveratrol, lutein, and CoQ10, induced autophagy and diminished the cytotoxic effects of ICG and BBG in ocular cells. These results suggest that autophagy may protect ARPE-19 and 661W cells from vital dyes-induced damage.
- Subjects :
- Photoreceptors
0301 basic medicine
Sensory Receptors
genetic structures
Ubiquinone
Cytotoxicity
Social Sciences
lcsh:Medicine
Retinal Pigment Epithelium
Resveratrol
Toxicology
Pathology and Laboratory Medicine
Mice
chemistry.chemical_compound
0302 clinical medicine
Animal Cells
Vitrectomy
Stilbenes
Rosaniline Dyes
Medicine and Health Sciences
Psychology
Cytotoxic T cell
Animal Anatomy
Coloring Agents
lcsh:Science
Cells, Cultured
Animal Ocular Anatomy
Cellular Stress Responses
Neurons
Multidisciplinary
Cell Death
Ophthalmic Procedures
Chloroquine
Cell biology
Cell Processes
Sensory Perception
Anatomy
Cellular Types
Research Article
Signal Transduction
Indocyanine Green
Programmed cell death
Cell Survival
Autophagic Cell Death
Ocular Anatomy
Immunoblotting
Molecular Probe Techniques
Surgical and Invasive Medical Procedures
Vitreoretinal Surgery
Protective Agents
Research and Analysis Methods
03 medical and health sciences
Ocular System
Autophagy
Animals
Humans
Viability assay
Molecular Biology Techniques
Molecular Biology
Lutein
lcsh:R
Biology and Life Sciences
Afferent Neurons
Retinal
Cell Biology
eye diseases
030104 developmental biology
chemistry
Cellular Neuroscience
030221 ophthalmology & optometry
lcsh:Q
Zoology
Indocyanine green
Neuroscience
Subjects
Details
- ISSN :
- 19326203
- Volume :
- 12
- Database :
- OpenAIRE
- Journal :
- PLOS ONE
- Accession number :
- edsair.doi.dedup.....ccc8339ce8c0c83eec6b7cc5469e910a