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Paper electrophoresis and chromatography of oligopeptides with N-terminal methionine
- Source :
- Analytical Biochemistry. 54:161-169
- Publication Year :
- 1973
- Publisher :
- Elsevier BV, 1973.
-
Abstract
- Several electrophoretic and chromatographic systems are described for the separation of di- and tripeptides with N-terminal methionine. The most effective methods of separation are electrophoresis in barbital-triethylamine-acetic acid, pH 8.0 and chromatography in n -butanol-acetic acid-water, 18:2:5. Increased resolution may be obtained by using two or more systems in combination. These methods were intended to separate and identify the oligopeptides formed during the initiation of eukaryotic protein synthesis, but may also be applied to the separation of small peptides generally.
- Subjects :
- Oligopeptide
Methionine
Chromatography
Resolution (mass spectrometry)
Chromatography, Paper
Chemistry
Biophysics
Cell Biology
Paper electrophoresis
Tripeptide
Biochemistry
chemistry.chemical_compound
Electrophoresis
Sulfur Isotopes
Small peptide
Methods
Protein biosynthesis
Electrophoresis, Paper
Amino Acid Sequence
Amino Acids
Oligopeptides
Oxidation-Reduction
Molecular Biology
Subjects
Details
- ISSN :
- 00032697
- Volume :
- 54
- Database :
- OpenAIRE
- Journal :
- Analytical Biochemistry
- Accession number :
- edsair.doi.dedup.....ccbcfaf5a768c28172696e5e4f6119c8