Back to Search
Start Over
Purine Catabolism Shows a Dampened Circadian Rhythmicity in a High-fat Diet-Induced Mouse Model of Obesity
- Source :
- Molecules, Volume 24, Issue 24
- Publication Year :
- 2019
- Publisher :
- MDPI, 2019.
-
Abstract
- High-calorie diet, circadian rhythms and metabolic features are intimately linked. However, the mediator(s) between nutritional status, circadian rhythms and metabolism remain largely unknown. This article aims to clarify the key metabolic pathways bridging nutritional status and circadian rhythms based on a combination of metabolomics and molecular biological techniques. A mouse model of high-fat diet-induced obesity was established and serum samples were collected in obese and normal mice at different zeitgeber times. Gas chromatography/mass spectrometry, multivariate/univariate data analyses and metabolic pathway analysis were used to reveal changes in metabolism. Metabolites involved in the metabolism of purines, carbohydrates, fatty acids and amino acids were markedly perturbed in accordance with circadian related variations, among which purine catabolism showed a typical oscillation. What&rsquo<br />s more, the rhythmicity of purine catabolism dampened in the high-fat diet group. The expressions of clock genes and metabolic enzymes in the liver were measured. The mRNA expression of Xanthine oxidase (Xor) was highly correlated with the rhythmicity of Clock, Rev-erb&alpha<br />and Bmal1, as well as the metabolites involved in purine catabolism. These data showed that a high-fat diet altered the circadian rhythm of metabolic pathways, especially purine catabolism. It had an obvious circadian oscillation and a high-fat diet dampened its circadian rhythmicity. It was suggested that circadian rhythmicity of purine catabolism is related to circadian oscillations of expression of Xor, Uox and corresponding clock genes.
- Subjects :
- Purine
medicine.medical_specialty
obesity
Pharmaceutical Science
030209 endocrinology & metabolism
Biology
Diet, High-Fat
Article
Analytical Chemistry
03 medical and health sciences
chemistry.chemical_compound
Mice
0302 clinical medicine
Internal medicine
Drug Discovery
medicine
Zeitgeber
Animals
Circadian rhythm
Physical and Theoretical Chemistry
Purine metabolism
Xanthine oxidase
030304 developmental biology
0303 health sciences
Catabolism
GC/MS
Organic Chemistry
purine catabolism
Metabolism
metabolomics
Circadian Rhythm
CLOCK
Disease Models, Animal
Endocrinology
high-fat diet
chemistry
Chemistry (miscellaneous)
circadian rhythms
Purines
Metabolome
Molecular Medicine
Biomarkers
Subjects
Details
- Language :
- English
- ISSN :
- 14203049
- Volume :
- 24
- Issue :
- 24
- Database :
- OpenAIRE
- Journal :
- Molecules
- Accession number :
- edsair.doi.dedup.....ccb5d7c74805d81601c460a0d99cd6bc