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Biodentine Reduces Tumor Necrosis Factor Alpha-induced TRPA1 Expression in Odontoblastlike Cells
- Source :
- El Karim, I A, McCrudden, M T C, McGahon, M K, Curtis, T M, Jeanneau, C, Giraud, T, Irwin, C R, Linden, G J, Lundy, F T & About, I 2016, ' Biodentine Reduces Tumor Necrosis Factor Alpha-induced TRPA1 Expression in Odontoblastlike Cells ', Journal of Endodontics, vol. 42, no. 4, pp. 589-595 . https://doi.org/10.1016/j.joen.2015.12.017, Journal of Endodontics, Journal of Endodontics, Elsevier, 2016, 42 (4), pp.589-595. ⟨10.1016/j.joen.2015.12.017⟩, Journal of Endodontics, 2016, 42 (4), pp.589-595. ⟨10.1016/j.joen.2015.12.017⟩
- Publication Year :
- 2016
-
Abstract
- International audience; Introduction: The transient receptor potential (TRP) ion channels have emerged as important cellular sensors in both neuronal and non-neuronal cells, with TRPA1 playing a central role in nociception and neurogenic inflammation. The functionality of TRP channels has been shown to be modulated by inflammatory cytokines. The aim of this study was to investigate the effect of inflammation on odontoblast TRPA1 expression and to determine the effect of Biodentine (Septodent, Paris, France) on inflammatory-induced TRPA1 expression. Methods: Immunohistochemistry was used to study TRPA1 expression in pulp tissue from healthy and carious human teeth. Pulp cells were differentiated to odontoblastlike cells in the presence of 2 mmol/L beta-glycerophosphate, and these cells were used in quantitative polymerase chain reaction, Western blotting, calcium imaging, and patch clamp studies. Results: Immunofluorescent staining revealed. TRPA1 expression in odontoblast cell bodies and odontoblast processes, which was more intense in carious versus healthy teeth. TRPA1 gene expression was induced in cultured odontoblastlike cells by tumor necrosis factor alpha, and this expression was significantly reduced in the presence of Biodentine. The functionality of the TRPA1 channel was shown by calcium microfluorimetry and patch clamp recording, and our results showed a significant reduction in tumor necrosis factor alpha induced TRPA1 responses after Biodentine treatment. Conclusions: In conclusion, this study showed TRPA1 to be modulated by caries-induced inflammation and that Biodentine reduced TRPA1 expression and functional responses.
- Subjects :
- 0301 basic medicine
Pathology
medicine.medical_specialty
Materials science
Cellular differentiation
[SDV]Life Sciences [q-bio]
TRPV Cation Channels
Nerve Tissue Proteins
Inflammation
Dental Caries
Dental Pulp Capping
Proinflammatory cytokine
03 medical and health sciences
Transient receptor potential channel
Transient Receptor Potential Channels
0302 clinical medicine
stomatognathic system
SDG 3 - Good Health and Well-being
medicine
Humans
Patch clamp
Odontoblast
TRPA1 Cation Channel
General Dentistry
Dental Pulp
Stem cell
Odontoblasts
Tumor Necrosis Factor-alpha
Dentistry(all)
Silicates
food and beverages
Cell Differentiation
030206 dentistry
Calcium Compounds
Immunohistochemistry
Molecular biology
3. Good health
Biodentine
030104 developmental biology
Glycerophosphates
Pulp (tooth)
Tumor necrosis factor alpha
Calcium Channels
medicine.symptom
psychological phenomena and processes
Subjects
Details
- Language :
- English
- ISSN :
- 00992399
- Database :
- OpenAIRE
- Journal :
- El Karim, I A, McCrudden, M T C, McGahon, M K, Curtis, T M, Jeanneau, C, Giraud, T, Irwin, C R, Linden, G J, Lundy, F T & About, I 2016, ' Biodentine Reduces Tumor Necrosis Factor Alpha-induced TRPA1 Expression in Odontoblastlike Cells ', Journal of Endodontics, vol. 42, no. 4, pp. 589-595 . https://doi.org/10.1016/j.joen.2015.12.017, Journal of Endodontics, Journal of Endodontics, Elsevier, 2016, 42 (4), pp.589-595. ⟨10.1016/j.joen.2015.12.017⟩, Journal of Endodontics, 2016, 42 (4), pp.589-595. ⟨10.1016/j.joen.2015.12.017⟩
- Accession number :
- edsair.doi.dedup.....ccafe4f8126bdde3dda5294f12762fff