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p62 : A Constitutively Tyrosine-Phosphorylated, GAP-Associated Protein in Chronic Myelogenous Leukemia Progenitor Cells
- Source :
- Cell. 88:197-204
- Publication Year :
- 1997
- Publisher :
- Elsevier BV, 1997.
-
Abstract
- Characteristic of chronic myelogenous leukemia (CML) is the presence of the chimeric p210(bcr-abl) protein possessing elevated protein tyrosine kinase activity relative to normal c-abl tyrosine kinase. Hematopoietic progenitors isolated from CML patients in the chronic phase contain a constitutively tyrosine-phosphorylated protein that migrates at 62 kDa by SDS-PAGE and associates with the p120 ras GTPase-activating protein (GAP). We have purified p62(dok) from a hematopoietic cell line expressing p210(bcr-abl). p62(dok) is a novel protein with features of a signaling molecule. Association of p62(dok) with GAP correlates with its tyrosine phosphorylation. p62(dok) is rapidly tyrosine-phosphorylated upon activation of the c-Kit receptor, implicating it as a component of a signal transduction pathway downstream of receptor tyrosine kinases.
- Subjects :
- Fusion Proteins, bcr-abl
Protein tyrosine phosphatase
General Biochemistry, Genetics and Molecular Biology
Receptor tyrosine kinase
CSK Tyrosine-Protein Kinase
src Homology Domains
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Leukemia, Myelogenous, Chronic, BCR-ABL Positive
hemic and lymphatic diseases
Tumor Cells, Cultured
Humans
CD135
Cloning, Molecular
Phosphorylation
Phosphotyrosine
030304 developmental biology
Stem Cell Factor
0303 health sciences
biology
Biochemistry, Genetics and Molecular Biology(all)
GTPase-Activating Proteins
Proteins
RNA-Binding Proteins
Tyrosine phosphorylation
Protein-Tyrosine Kinases
Hematopoietic Stem Cells
Phosphoproteins
Cell biology
DNA-Binding Proteins
Proto-Oncogene Proteins c-kit
src-Family Kinases
chemistry
ras GTPase-Activating Proteins
ROR1
biology.protein
Electrophoresis, Polyacrylamide Gel
GRB2
Tyrosine kinase
Signal Transduction
030215 immunology
Proto-oncogene tyrosine-protein kinase Src
Subjects
Details
- ISSN :
- 00928674
- Volume :
- 88
- Database :
- OpenAIRE
- Journal :
- Cell
- Accession number :
- edsair.doi.dedup.....cc96591f54328ee2d2ba19048db9f567