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RNA silencing of rotavirus gene expression
- Source :
- Virus Research
- Publication Year :
- 2004
- Publisher :
- Elsevier BV, 2004.
-
Abstract
- RNA interference (RNAi) is a double-stranded RNA (dsRNA)-triggered mechanism for suppressing gene expression, which is conserved in evolution and has emerged as a powerful tool to study gene function. Rotaviruses, the leading cause of severe diarrhea in young children, are formed by three concentric layers of protein, and a genome composed of 11 segments of dsRNA. Here, we show that the RNAi machinery can be triggered to silence rotavirus gene expression by sequence-specific short interfering RNAs (siRNAs). RNAi is also useful for the study of the virus-cell interactions, through the silencing of cellular genes that are potentially important for the replication of the virus. Interestingly, while the translation of mRNAs is readily stopped by the RNAi machinery, the viral transcripts involved in virus genome replication do not seem to be susceptible to RNAi. Since gene silencing by RNAi is very efficient and specific, this system could become a novel therapeutic approach for rotavirus and other virus infections, once efficient methods for in vivo delivery of siRNAs are developed. Although the use of RNAi as an antiviral therapeutic tool remains to be demonstrated, there is no doubt that this technology will influence drastically the way postgenomic virus research is conducted.
- Subjects :
- Gene Expression Regulation, Viral
Rotavirus
Cancer Research
Small interfering RNA
Genes, Viral
RNA-induced transcriptional silencing
RNA-induced silencing complex
RNA Stability
viruses
Trans-acting siRNA
Biology
Reoviridae
Article
RNA interference
DNA-directed RNA interference
Virology
Gene silencing
RNA, Small Interfering
fungi
siRNAs
RNA silencing
Antiviral agents
Infectious Diseases
RNA, Viral
Subjects
Details
- ISSN :
- 01681702
- Volume :
- 102
- Database :
- OpenAIRE
- Journal :
- Virus Research
- Accession number :
- edsair.doi.dedup.....cc6bbef52419dddc473d862ddca73109