Supplementary Figures 1 through 6 from Small-Molecule Inhibitors of USP1 Target ID1 Degradation in Leukemic Cells

PDF - 502KB, Supplementary figure 1. USP1 inhibitor SJB2-043 inhibits activity of native USP1 in a dose-dependent manner and causes a decrease in ID1, ID2 and ID3 levels in K562 cells. Supplementary figure 2. Knockdown of USP1 results in growth inhibition, increased apoptosis and cell cycle arrest in K562 cells. Supplementary figure 3. Analogs of USP1 inhibitor lead to ID1 destabilization in leukemic cells. Supplementary figure 4. Caspase inhibitor does not block the degradation of ID1 by USP1 inhibitors. Supplementary figure 5. Effects of USP1 inhibitors on growth of primary human cord blood CD34+ cells. Supplementary figure 6. USP1 inhibitors cause increase in Ub-FANCD2, Ub-PCNA, and p21, and decrease the HR activity.