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Serotonin release and cell proliferation are under the control of alpha-bungarotoxin-sensitive nicotinic receptors in small-cell lung carcinoma cell lines
- Source :
- FEBS letters. 342(3)
- Publication Year :
- 1994
-
Abstract
- Neuronal type nicotinic acetylcholine receptors (nAchRs) have recently been identified in small-cell lung carcinoma. We here show that both nicotine and cytisine stimulate [3H]serotonin release in a dose-dependent manner; this effect is antagonized by alpha-bungarotoxin (alpha Bgtx) and alpha-conotoxin MI (alpha Ctx). Nicotine and cytisine stimulate in vitro SCLC proliferation and this effect is completely antagonized by both alpha Bgtx and alpha Ctx. By PCR analysis, we demonstrate the presence in SCLC of both the alpha 7 and the beta 2 nAchR subunits mRNA. These data show that nAchRs play an important role in the biology of SCLC, and that alpha Bgtx-sensitive receptors of the alpha 7 subtype are crucially involved in both the secretagogue and mitogenic effects of nicotinic agonists.
- Subjects :
- medicine.medical_specialty
Serotonin
Lung Neoplasms
Nicotinic acetylcholine receptor
Proliferation
Molecular Sequence Data
Biophysics
Gene Expression
Pharmacology
Biology
Receptors, Nicotinic
complex mixtures
Biochemistry
Nicotine
Cytisine
chemistry.chemical_compound
Structural Biology
Internal medicine
Genetics
medicine
Tumor Cells, Cultured
Humans
RNA, Messenger
Carcinoma, Small Cell
Receptor
Molecular Biology
Secretion
Acetylcholine receptor
DNA Primers
Base Sequence
Cell Biology
Bungarotoxin
Bungarotoxins
respiratory tract diseases
Endocrinology
Nicotinic agonist
nervous system
chemistry
Secretory Rate
Human small cell lung carcinoma
Cell Division
medicine.drug
Subjects
Details
- ISSN :
- 00145793
- Volume :
- 342
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- FEBS letters
- Accession number :
- edsair.doi.dedup.....cc30a163e5659e0a33011d121da5ff14