Back to Search
Start Over
Effects of a catechin-free fraction derived from green tea on gene expression of enzymes related to lipid metabolism in the mouse liver
- Source :
- Biomedical Research. 33:9-13
- Publication Year :
- 2012
- Publisher :
- Biomedical Research Press, 2012.
-
Abstract
- Many biological activities of green tea have been attributed to a major constituent, (minus;)-epigallocatechin gallate (EGCG). We previously reported that EGCG and an EGCG-free fraction derived from green tea modulated the gene expression of gluconeogenic enzymes, glucose-6-phosphatase and phosphoenolpyruvate carboxykinase, in the mouse liver. EGCG is also known to affect the gene expression of enzymes related to lipid metabolism. However, it remains to be examined whether or not a constituent other than EGCG contributes to the change in gene expression of these enzymes. In this study, we prepared an EGCG-free water-soluble fraction (GT-W), and examined its effects on the hepatic gene expression of lipogenic enzymes in mice. The results of quantitative real-time PCR assays indicated that the dietary administration of GT-W for 4 weeks reduced the hepatic gene expression of lipogenic enzymes: fatty acid synthase, hydroxymethylglutaryl coenzyme A reductase, and acetyl-coenzyme A carboxylase alpha. Also, the gene expression of sterol regulatory element-binding transcription factor (Srebf)1 and/or Srebf2 was reduced, suggesting that the reduction of Srebfs contributed to the down-regulation of the lipogenic enzymes, since these transcription factors bind the promoter region to enhance their expression. The plasma levels of triglycerides and cholesterol were reduced with statistical significance in the group given a diet containing GT-W. These results suggest that in addition to EGCG, green tea contains some component(s) which may help to prevent arteriosclerosis and obesity.
- Subjects :
- Blood Glucose
Male
Arteriosclerosis
Coenzyme A
Down-Regulation
Real-Time Polymerase Chain Reaction
complex mixtures
Catechin
General Biochemistry, Genetics and Molecular Biology
Mice
chemistry.chemical_compound
Gene expression
Animals
Obesity
Triglycerides
chemistry.chemical_classification
Mice, Inbred BALB C
Tea
biology
Plant Extracts
food and beverages
Lipid metabolism
Promoter
General Medicine
Lipid Metabolism
Molecular biology
Plant Leaves
Fatty acid synthase
Cholesterol
Enzyme
Liver
chemistry
Biochemistry
Glucose-6-Phosphatase
biology.protein
sense organs
Sterol Regulatory Element Binding Protein 1
Phosphoenolpyruvate carboxykinase
Phosphoenolpyruvate Carboxykinase (ATP)
Sterol Regulatory Element Binding Protein 2
Subjects
Details
- ISSN :
- 1880313X and 03886107
- Volume :
- 33
- Database :
- OpenAIRE
- Journal :
- Biomedical Research
- Accession number :
- edsair.doi.dedup.....cc290a0d9e87eb8938e7ef26ac8369a5