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7,8-Dihydroxyflavone suppresses proliferation and induces apoptosis of human osteosarcoma cells
- Source :
- Acta Biochimica et Biophysica Sinica. 53:903-911
- Publication Year :
- 2021
- Publisher :
- China Science Publishing & Media Ltd., 2021.
-
Abstract
- Recent studies suggest that 7,8-dihydroxyflavone (7,8-DHF) inhibits the development of several tumors. However, its role in osteosarcoma (OS) remains unknown. This study was designed to investigate the effects and underlying mechanisms of 7,8-DHF that may influence OS development. Human OS cell lines (U2OS and 143B) were treated with 7,8-DHF; cell viability and cell migration were assessed by methylthiazolyldiphenyl-tetrazolium bromide (MTT) assay and wound-healing assay, respectively; and cell death and apoptosis were evaluated by LIVE/DEAD staining and terminal deoxynucleotidyl transferase (TdT) dUTP nick-end labeling (TUNEL) assay, respectively. Reactive oxygen species production was measured using 2,7-dichlorodihydrofluorescein diacetate probe. Akt, Bcl-xL/Bcl-2 asociated death promoter (Bad), p38 mitogen-activated protein kinase (MAPK), extracellular regulated protein kinase (ERK), and c-Jun N-terminal kinase (JNK) expression and their respective phosphorylation levels were detected by western blot analysis. We found that 7,8-DHF reduced cell viability in a dose-dependent manner and also promoted apoptosis, inhibited migration, and induced oxidative stress in OS cells. Moreover, 7,8-DHF inhibited Akt, Bad, and p38MAPK, but activated ERK and JNK signals. In summary, our results suggest that 7,8-DHF inhibits OS progression, possibly by regulating Akt/Bad and MAPK signaling. These findings provide new evidence for the pharmacological effects of 7,8-DHF that may improve drug therapy for OS patients.
- Subjects :
- 0301 basic medicine
MAPK/ERK pathway
Osteosarcoma
Programmed cell death
TUNEL assay
Chemistry
p38 mitogen-activated protein kinases
Biophysics
Apoptosis
Bone Neoplasms
General Medicine
Flavones
Biochemistry
Molecular biology
03 medical and health sciences
030104 developmental biology
0302 clinical medicine
Terminal deoxynucleotidyl transferase
Cell Line, Tumor
030220 oncology & carcinogenesis
Humans
Viability assay
Protein kinase A
Protein kinase B
Cell Proliferation
Subjects
Details
- ISSN :
- 16729145
- Volume :
- 53
- Database :
- OpenAIRE
- Journal :
- Acta Biochimica et Biophysica Sinica
- Accession number :
- edsair.doi.dedup.....cc1907e4baea7fac8dcdd49b26696cef