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Macrophages facilitate electrical conduction in the heart
- Source :
- Cell, vol 169, iss 3
- Publication Year :
- 2017
- Publisher :
- Elsevier BV, 2017.
-
Abstract
- Organ-specific functions of tissue-resident macrophages in the steady-state heart are unknown. Here, we show that cardiac macrophages facilitate electrical conduction through the distal atrioventricular node, where conducting cells densely intersperse with elongated macrophages expressing connexin 43. When coupled to spontaneously beating cardiomyocytes via connexin-43-containing gap junctions, cardiac macrophages have a negative resting membrane potential and depolarize in synchrony with cardiomyocytes. Conversely, macrophages render the resting membrane potential of cardiomyocytes more positive and, according to computational modeling, accelerate their repolarization. Photostimulation of channelrhodopsin-2-expressing macrophages improves atrioventricular conduction, whereas conditional deletion of connexin 43 in macrophages and congenital lack of macrophages delay atrioventricular conduction. In the Cd11b(DTR) mouse, macrophage ablation induces progressive atrioventricular block. These observations implicate macrophages in normal and aberrant cardiac conduction.
- Subjects :
- 0301 basic medicine
computational modeling
Male
Connexin
atrioventricular node
Inbred C57BL
Cardiovascular
Medical and Health Sciences
connexin 43
Mice
gap-junctions
2.1 Biological and endogenous factors
Myocyte
Myocytes, Cardiac
single-cell RNA-sequencing
Aetiology
steady-state
Gap junction
electrical conduction
Anatomy
11 Medical And Health Sciences
Biological Sciences
Middle Aged
Atrioventricular node
myocardial-infarction
Cell biology
Heart Disease
medicine.anatomical_structure
cardiovascular system
Female
Electrical conduction system of the heart
Cardiac
Life Sciences & Biomedicine
tissue macrophages
Biochemistry & Molecular Biology
tissue clearing
heart
Biology
General Biochemistry, Genetics and Molecular Biology
03 medical and health sciences
Heart Conduction System
Cardiac conduction
cardiac macrophages
medicine
Repolarization
Animals
Humans
Heart Atria
optogenetics
gap junctions
Myocytes
Science & Technology
membrane channel
Macrophages
Cell Biology
06 Biological Sciences
myocytes
medicine.disease
Mice, Inbred C57BL
030104 developmental biology
immune-system
cells
Atrioventricular block
Developmental Biology
Subjects
Details
- Database :
- OpenAIRE
- Journal :
- Cell, vol 169, iss 3
- Accession number :
- edsair.doi.dedup.....cbfdaa16e999a689bd73ac5b4eb0b953