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Spatial-temporal profiling of prodiginines and serratamolides produced by endophytic Serratia marcescens harbored in Maytenus serrata
- Source :
- Scientific Reports, Vol 8, Iss 1, Pp 1-15 (2018), Scientific Reports
- Publication Year :
- 2018
- Publisher :
- Nature Publishing Group, 2018.
-
Abstract
- An endophytic bacterium, Serratia marcescens MSRBB2, isolated from inner bark of a Cameroonian Maytenus serrata plant, was subjected to the OSMAC (One Strain Many Compounds) approach and metabolic profiling using HPLC-HRMSn. We identified 7 prodiginines along with 26 serratamolides. Their biosynthetic pathways were elucidated by feeding with labeled precursors in combination with HRMSn. Dual-culture confrontation/restriction assays of the bacterial endophyte were devised with coexisting fungal endophytes (Pestalotiopsis virgatula, Aspergillus caesiellus and Pichia spp.) as well as with unrelated, non-endophytic fungi belonging to the same genera. The assays were combined with scanning electron microscopy (SEM) as well as matrix-assisted laser desorption ionization imaging high-resolution mass spectrometry (MALDI-imaging-HRMS) for visualizing, both in high spatial and temporal resolution, the distribution and interplay of the compounds during microbial interactions. We demonstrated the effect of prodigiosin produced by endophytic S. marcescens MSRBB2 as an allelochemical that specifically inhibits coexisting endophytic fungi. Our results provide new insights into the physiological and ecological relevance of prodiginines and serratamolides within the context of allelopathy and chemical defense interaction occurring between coexisting endophytes harbored in M. serrata.
- Subjects :
- 0301 basic medicine
030106 microbiology
lcsh:Medicine
Endophyte
Article
Plant use of endophytic fungi in defense
Microbiology
Prodigiosin
03 medical and health sciences
chemistry.chemical_compound
Depsipeptides
Endophytes
lcsh:Science
Chromatography, High Pressure Liquid
Serratia marcescens
Allelopathy
Pichia
Multidisciplinary
biology
Maytenus
lcsh:R
biology.organism_classification
Biosynthetic Pathways
030104 developmental biology
chemistry
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Metabolome
Microbial Interactions
lcsh:Q
Bacteria
Subjects
Details
- Language :
- English
- ISSN :
- 20452322
- Volume :
- 8
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Scientific Reports
- Accession number :
- edsair.doi.dedup.....cbf2d6e9a160f7b32aab20e5a18d4b41