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Effects of acute agonist treatment on subcellular distribution of κ opioid receptor in rat spinal cord
- Source :
- Journal of Neuroscience Research. 87:1695-1702
- Publication Year :
- 2009
- Publisher :
- Wiley, 2009.
-
Abstract
- We investigated whether acute treatment with agonists affected the subcellular distribution of kappa opioid receptor (KOPR) in the dorsal horn of the rat lumbar spinal cord by using immunoelectron microscopy. Rats were injected intrathecally (i.t.) with U50,488H (100 nmole), dynorphin A(1-17) (15 nmole), or vehicle. The doses chosen have been shown to induce antinociception. Rats were perfused transcardially 30 min later, and lumbar spinal cords were removed and processed for electron microscopic analysis. KOPR was stained with KT-2, a specific polyclonal antibody against the rat/mouse KOPR(371-380) peptide, followed by gold-labeled secondary antibody and silver intensification. The silver grains were present in axons, terminals, dendrites, and somata, and the association with plasma membranes was quantified in dendrites, because KOPR immunoreactivity was most frequently observed in these profiles. In vehicle-treated rats, approximately 27% of KOPR immunoreactivity was associated with plasma membranes. U50,488H, i.t., did not cause a significant change in the percentage of KOPR present on plasma membranes, whereas dynorphin A, i.t., significantly decreased cell surface KOPR to approximately 19%. In summary, these data indicate that U50,488H and dynorphin A differentially regulate the subcellular distribution of endogenous KOPR.
- Subjects :
- Male
Agonist
medicine.medical_specialty
medicine.drug_class
Immunoelectron microscopy
Gene Expression
Dynorphin
Dynorphins
Fluorescence
Article
Catheterization
Cell Line
Rats, Sprague-Dawley
Cellular and Molecular Neuroscience
chemistry.chemical_compound
Opioid receptor
Internal medicine
medicine
Animals
Humans
Microscopy, Immunoelectron
Posterior Horn Cell
Injections, Spinal
Analysis of Variance
Lumbar Vertebrae
Chemistry
Receptors, Opioid, kappa
Cell Membrane
3,4-Dichloro-N-methyl-N-(2-(1-pyrrolidinyl)-cyclohexyl)-benzeneacetamide, (trans)-Isomer
Dynorphin A
Anatomy
Spinal cord
Immunohistochemistry
Rats
Posterior Horn Cells
Lumbar Spinal Cord
medicine.anatomical_structure
Endocrinology
Spinal Cord
Subjects
Details
- ISSN :
- 10974547 and 03604012
- Volume :
- 87
- Database :
- OpenAIRE
- Journal :
- Journal of Neuroscience Research
- Accession number :
- edsair.doi.dedup.....cbde03c210a00d6f01d47ad66f9f2945