Back to Search
Start Over
Micro-staining microbes: An alternative to traditional staining of microbiological specimens using microliter volumes of reagents
- Source :
- Journal of microbiological methods. 164
- Publication Year :
- 2019
-
Abstract
- Microbial staining techniques are widely employed in clinical and academic laboratories for classifying and identifying microorganisms derived from clinical, food and environmental samples. Staining allows for the rapid visualization and determination of many morphological characteristics of microorganisms, used for their identification and classification. Over the past century, staining techniques such as the Gram stain, the Capsule stain, the Acid-fast stain and the Endospore stain, have seen few advances, and manual staining remains the gold standard. Typical instructions for these staining procedures recommend 'flooding' glass slides with milliliter volumes of dye, resulting in large volumes of hazardous waste. Here we present micro-staining, a simple alternative to flooding that utilizes microliter volumes of dye. Micro-staining minimizes the volume of waste generated, leads to significant cost savings for the laboratory, requires limited training, and produces results with equivalent quality to traditional stains.
- Subjects :
- Microbiology (medical)
Microbiological Techniques
Spores
Microbiology
Endospore
Stain
law.invention
03 medical and health sciences
law
Humans
Microbial staining
Coloring Agents
Molecular Biology
Bacterial Capsules
030304 developmental biology
0303 health sciences
Chromatography
Bacteria
Staining and Labeling
030306 microbiology
Chemistry
Cost savings
Staining
Gram staining
Acid-fast
Phenazines
Gentian Violet
Indicators and Reagents
Subjects
Details
- ISSN :
- 18728359
- Volume :
- 164
- Database :
- OpenAIRE
- Journal :
- Journal of microbiological methods
- Accession number :
- edsair.doi.dedup.....cbc4f2bc6bf6480554f41a24fc5a7780