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Cellular responses to beating hydrogels to investigate mechanotransduction
- Source :
- Nature Communications, Vol 10, Iss 1, Pp 1-13 (2019), Nature Communications
- Publication Year :
- 2019
- Publisher :
- Nature Publishing Group, 2019.
-
Abstract
- Cells feel the forces exerted on them by the surrounding extracellular matrix (ECM) environment and respond to them. While many cell fate processes are dictated by these forces, which are highly synchronized in space and time, abnormal force transduction is implicated in the progression of many diseases (muscular dystrophy, cancer). However, material platforms that enable transient, cyclic forces in vitro to recreate an in vivo-like scenario remain a challenge. Here, we report a hydrogel system that rapidly beats (actuates) with spatio-temporal control using a near infra-red light trigger. Small, user-defined mechanical forces (~nN) are exerted on cells growing on the hydrogel surface at frequencies up to 10 Hz, revealing insights into the effect of actuation on cell migration and the kinetics of reversible nuclear translocation of the mechanosensor protein myocardin related transcription factor A, depending on the actuation amplitude, duration and frequency.<br />Mechanotransduction of cells is of interest for a number of reasons but model in vitro systems remain a challenge. Here, the authors report on a hydrogel which changes properties upon near infrared irradiation to create cyclic forces and demonstrate the application of these gels to study mechanotransduction.
- Subjects :
- 0301 basic medicine
Polymers
Science
Active Transport, Cell Nucleus
General Physics and Astronomy
02 engineering and technology
Cell fate determination
Mechanotransduction, Cellular
Article
General Biochemistry, Genetics and Molecular Biology
Cell Line
Biomaterials
Extracellular matrix
Mice
03 medical and health sciences
Cell Movement
Animals
Cell migration
Mechanotransduction
lcsh:Science
Transcription factor
Cytoskeleton
Cell Nucleus
Multidisciplinary
Chemistry
Hydrogels
General Chemistry
Fibroblasts
021001 nanoscience & nanotechnology
Actins
Extracellular Matrix
Kinetics
030104 developmental biology
Myocardin
Self-healing hydrogels
Trans-Activators
Biophysics
lcsh:Q
Gels and hydrogels
0210 nano-technology
Transduction (physiology)
Cell signalling
Subjects
Details
- Language :
- English
- ISSN :
- 20411723
- Volume :
- 10
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Nature Communications
- Accession number :
- edsair.doi.dedup.....cb9c216fa743a652f37d0f5ee22c8aa4