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Sensitive and rapid on-site detection of SARS-CoV-2 using a gold nanoparticle-based high-throughput platform coupled with CRISPR/Cas12-assisted RT-LAMP
- Source :
- Sensors and Actuators. B, Chemical
- Publication Year :
- 2021
- Publisher :
- Elsevier BV, 2021.
-
Abstract
- Graphical abstract<br />The outbreak of corona virus disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has led to a global pandemic. The high infectivity of SARS-CoV-2 highlights the need for sensitive, rapid and on-site diagnostic assays of SARS-CoV-2 with high-throughput testing capability for large-scale population screening. The current detection methods in clinical application need to operate in centralized labs. Though some on-site detection methods have been developed, few tests could be performed for high-throughput analysis. We here developed a gold nanoparticle-based visual assay that combines with CRISPR/Cas12a-assisted RT-LAMP, which is called Cas12a-assisted RT-LAMP/AuNP (CLAP) assay for rapid and sensitive detection of SARS-CoV-2. In optimal condition, we could detect down to 4 copies/μL of SARS-CoV-2 RNA in 40 min. by naked eye. The sequence-specific recognition character of CRISPR/Cas12a enables CLAP a superior specificity. More importantly, the CLAP is easy for operation that can be extended to high-throughput test by using a common microplate reader. The CLAP assay holds a great potential to be applied in airports, railway stations, or low-resource settings for screening of suspected people. To the best of our knowledge, this is the first AuNP-based colorimetric assay coupled with Cas12 and RT-LAMP for on-site diagnosis of COVID-19. We expect CLAP assay will improve the current COVID-19 screening efforts, and make contribution for control and mitigation of the pandemic.
- Subjects :
- Gold nanoparticle
Coronavirus disease 2019 (COVID-19)
Computer science
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)
RPA, recombinase polymerase amplification
NMPA, the Chinese National Medical Products Administration
Loop-mediated isothermal amplification
AuNP, gold nanoparticle
COVID-19, Corona Virus Disease 2019
Computational biology
Virus diseases
LAMP, loop-mediated isothermal amplification
SARS-CoV-2, severe acute respiratory syndrome coronavirus 2
Article
DMEM, Dulbecco’s modified Eagle’s medium
CRISPR/Cas
HCRs, hybridization chain reactions
Materials Chemistry
POCT, point of care testing
CRISPR
TCEP, Tris(2-carboxyethyl) phosphine
Electrical and Electronic Engineering
TEM, transmission electron microscopy
Instrumentation
Throughput (business)
ComputingMethodologies_COMPUTERGRAPHICS
FDA, American Food and Drug Administration
High-throughput on-site detection
Metals and Alloys
Condensed Matter Physics
Coronavirus disease
Surfaces, Coatings and Films
Electronic, Optical and Magnetic Materials
Microplate Reader
CRISPR, clustered regularly interspaced short palindromic repeats
Population screening
RT-qPCR, reverse transcription-real time quantitative PCR
Subjects
Details
- ISSN :
- 09254005
- Volume :
- 345
- Database :
- OpenAIRE
- Journal :
- Sensors and Actuators B: Chemical
- Accession number :
- edsair.doi.dedup.....cb96fd49cea3aea17a19a38f801ee651