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pl9skp1 and p45skp2 are essential elements of the cyclin A-CDK2 S phase kinase
- Source :
- Cell. 82(6):915-925
- Publication Year :
- 1995
- Publisher :
- Elsevier BV, 1995.
-
Abstract
- In normal human fibroblasts, cyclin A-CDK2 exists in a quaternary complex that contains p21 and PCNA. In many transformed cells, p21 disappears, and a substantial fraction of cyclin A-CDK2 complexes with p9ck1/cks2, p19 and p45. To investigate the significance of these rearrangements, we have isolated cDNAs encoding p19 and p45. In vitro reconstitution demonstrated that binding of p19 to cyclin A-CDK2 requires p45. Addition of these proteins to the kinase had no substantial effect on the kinase activity in vitro. Interference with p45 function in vivo by microinjection of antibodies or antisense oligonucleotides prevented entry into S phase in both normal and transformed cells. Cyclin A-CDK2 has previously been identified as a kinase whose activity is essential for S phase. Our results identify p45 as an essential element of this activity. The abundance of p45 is greatly increased in many transformed cells. This could result in changes in cell cycle control that contribute to the process of cellular transformation.
- Subjects :
- 0303 health sciences
Cyclin-dependent kinase 1
Biochemistry, Genetics and Molecular Biology(all)
Cyclin D
Cyclin A
Cyclin-dependent kinase 2
Cyclin-dependent kinase 3
Biology
Molecular biology
General Biochemistry, Genetics and Molecular Biology
03 medical and health sciences
0302 clinical medicine
Cyclin-dependent kinase
030220 oncology & carcinogenesis
biology.protein
Cyclin-dependent kinase complex
Cyclin A2
030304 developmental biology
Subjects
Details
- ISSN :
- 00928674
- Volume :
- 82
- Issue :
- 6
- Database :
- OpenAIRE
- Journal :
- Cell
- Accession number :
- edsair.doi.dedup.....cb84f7c5e02e127239623915e8ca22f5
- Full Text :
- https://doi.org/10.1016/0092-8674(95)90271-6