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Generation of two human isogenic iPSC lines from fetal dermal fibroblasts
- Source :
- Stem Cell Research, Vol 33, Iss, Pp 120-124 (2018), Stem Cell Research
- Publication Year :
- 2018
- Publisher :
- Elsevier, 2018.
-
Abstract
- Two isogenic hiPSC lines, ZIPi013-B and ZIPi013-E, were generated by reprogramming fetal dermal fibroblasts with episomal vectors. Previously, the same fetal fibroblasts were reprogrammed multiple times in a study comparing other reprogramming methods. As a consequence, the genomes have been sequenced multiple times. Both new cell lines offer the opportunity to study basic stem cell biology and model human disease. They can be applied as reference cell lines for creating isogenic clones bearing disease mutations on a well-characterized genomic background, as both cell lines have demonstrated excellent differentiation capacity in multiple labs. Resource table Unique stem cell lines identifier ZIPi013-B ZIPi013-E Alternative names of stem cell lines ZIP13K2 (ZIPi013-B) ZIP13K5 (ZIPi013-E) Institution Zentrum fur Integrative Psychiatrie gGmbH, Kiel, Germany Contact information of distributor PD Dr. Franz-Josef Muller, franz-josef.mueller@uksh.de Type of cell lines iPSC Origin human Cell Source Fibroblasts Clonality Clonal Method of reprogramming Transgene free, episomal Multiline rationale Isogenic clones Gene modification NO Type of modification N/A Associated disease N/A Gene/locus N/A Method of modification N/A Name of transgene or resistance N/A Inducible/constitutive system N/A Date archived/stock date Stock date ZIPi013-B 8th December 2017, stock date ZIPi013-E 12th December 2017 Cell line repository/bank N/A Ethical approval https://www.sciencellonline.com/technical-support/ethical-statement.html Ethikkommission der medizinischen Fakultat der Christian-Albrechts-Universitat zu Kiel, approval number A145/11
- Subjects :
- 0301 basic medicine
Fetus
Transgene
Induced Pluripotent Stem Cells
Medizin
Cell Differentiation
Locus (genetics)
Cell Biology
General Medicine
Fibroblasts
Biology
Molecular biology
Genome
03 medical and health sciences
030104 developmental biology
lcsh:Biology (General)
Cell culture
Humans
Female
Gene
Reprogramming
Stem cell biology
lcsh:QH301-705.5
Developmental Biology
Subjects
Details
- Language :
- English
- ISSN :
- 18735061
- Volume :
- 33
- Database :
- OpenAIRE
- Journal :
- Stem Cell Research
- Accession number :
- edsair.doi.dedup.....cb7664eb8601fbaed3bef930b6b345cf