Chromatographic Analyses, In Vitro Biological Activities, and Cytotoxicity of Cannabis sativa L. Essential Oil: A Multidisciplinary Study

WOS: 000454523000216
PubMed: 30544765
Due to renewed interest in the cultivation and production of Italian Cannabis sativa L., we proposed a multi-methodological approach to explore chemically and biologically both the essential oil and the aromatic water of this plant. We reported the chemical composition in terms of cannabinoid content, volatile component, phenolic and flavonoid pattern, and color characteristics. Then, we demonstrated the ethnopharmacological relevance of this plant cultivated in Italy as a source of antioxidant compounds toward a large panel of enzymes (pancreatic lipase, -amylase, -glucosidase, and cholinesterases) and selected clinically relevant, multidrug-sensible, and multidrug-resistant microbial strains (Staphylococcus aureus, Helicobacter pylori, Candida, and Malassezia spp.), evaluating the cytotoxic effects against normal and malignant cell lines. Preliminary in vivo cytotoxicity was also performed on Galleria mellonella larvae. The results corroborate the use of this natural product as a rich source of important biologically active molecules with particular emphasis on the role exerted by naringenin, one of the most important secondary metabolites.
Regione Lazio [13/2008]; MIUR FFABR2017 grants; Abruzzo District project "Filiera Regionale delle Piante Officinali"; Enrico and Enrica Sovena Foundation; Regione Lazio (La Canapa industriale: sviluppo e valorizzazione di una nuova filiera agroalimentare ecosostenibile, Italy) [CUP: B86C1800073002, 85201715069]
This work was supported by Regione Lazio (L.R. 13/2008-Progetti di Gruppi di Ricerca; La Canapa industriale: sviluppo e valorizzazione di una nuova filiera agroalimentare ecosostenibile, CUP: B86C1800073002, protocol number 85201715069, Italy) and by MIUR FFABR2017 grants held by Rossella Grande. The experimental work was supported by the Abruzzo District project "Filiera Regionale delle Piante Officinali". Antonella Di Sotto was supported by the "Enrico and Enrica Sovena Foundation".