Back to Search
Start Over
Rsf1p, a protein required for respiratory growth of Saccharomyces cerevisiae
- Source :
- Current Genetics. 43:263-272
- Publication Year :
- 2003
- Publisher :
- Springer Science and Business Media LLC, 2003.
-
Abstract
- A central problem in our understanding of mitochondrial (mt) function remains the question of how coordinate transcriptional control is accomplished between nucleus and mitochondria. Here, we report the initial characterization of a protein of previously unknown function, the product of the YMR030 W gene, that appears to mediate such coordinate gene expression. Expression of YMR030 W is glucose-repressible; a deletion mutant for this gene shows a severe growth defect on glycerol-, but not glucose- or ethanol-based medium. In that mutant, transcript levels from GUT1 and GUT2 are highly attenuated compared with those of the wild-type parent when both are grown on glycerol-based medium. Under the same growth conditions, transcripts from the mt OLI1 gene, which has one copy of a mt upstream activating sequence (UAS) in its 5'-flanking region, are attenuated in the DeltaYMR030 W mutant, but mRNA from the mt COX3 ( OXI2) gene, which lacks the mt UAS, are not. Some nuclear genes encoding mt-related proteins also show low transcript levels in the DeltaYMR030 W mutant in comparison with those of the wild-type parent strain during glycerol-based growth. Localization of the protein, via its expression fused to green fluorescent protein, indicates that it is present in both nucleus and mitochondria, supporting a respiration-related transcriptional role for this gene product in both cellular genetic compartments. Because of its role in both respiratory growth and mt function, we designate the YMR030 W coding sequence RSF1 (respiration factor 1).
- Subjects :
- Glycerol
Saccharomyces cerevisiae Proteins
Time Factors
Transcription, Genetic
Genes, Fungal
Green Fluorescent Proteins
Mutant
Saccharomyces cerevisiae
Biology
Fungal Proteins
Gene product
Upstream activating sequence
Gene Expression Regulation, Fungal
Two-Hybrid System Techniques
Gene expression
Genetics
Transcriptional regulation
RNA, Messenger
Gene
HSPA9
Cell Nucleus
Regulation of gene expression
Ethanol
Models, Genetic
Reverse Transcriptase Polymerase Chain Reaction
DNA
General Medicine
Molecular biology
Mitochondria
Luminescent Proteins
Glucose
Fermentation
Mutation
Gene Deletion
Plasmids
Transcription Factors
Subjects
Details
- ISSN :
- 14320983 and 01728083
- Volume :
- 43
- Database :
- OpenAIRE
- Journal :
- Current Genetics
- Accession number :
- edsair.doi.dedup.....cb5c776b3759e1efbebfedd2ba5246c3