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Genetic interactions of ribosome maturation factors Yvh1 and Mrt4 influence mRNA decay, glycogen accumulation, and the expression of early meiotic genes in Saccharomyces cerevisiae
- Source :
- Journal of Biochemistry. 150:103-111
- Publication Year :
- 2011
- Publisher :
- Oxford University Press (OUP), 2011.
-
Abstract
- The Saccharomyces cerevisiae Yvh1, a dual-specificity protein phosphatase involved in glycogen accumulation and sporulation, is required for normal vegetative growth. To further elucidate the role of Yvh1, we generated dominant mutants suppressing the slow growth caused by YVH1 disruption. One of the mutant alleles, designated as SVH1-1 (suppressor of Δyvh1 deletion), was identical to MRT4 (mRNA turnover) that contained a single-base substitution causing an amino acid change from Gly(68) to Asp. Mrt4(G68D) restored the deficiencies in growth and rRNA biogenesis that occurs in absence of Yvh1. Here, we report that the interaction between Mrt4 and Yvh1 is also essential for normal glycogen accumulation and mRNA decay as well as the induction of sporulation genes IME2, SPO13 and HOP1. The Mrt4(G68D) could restore the plethora of phenotypes we observed in absence of Yvh1. We found that Yvh1 is not essential for wild-type induction of the transcriptional regulator of these genes, IME1, suggesting that either translation or post-translational modification to activate Ime1 has been compromised. Since a defect in ribosome biogenesis in general can be related to other various defects, the ribosome biogenesis defect caused by absence of Yvh1 might be an indirect cause of observed phenotypes.
- Subjects :
- Ribosomal Proteins
Saccharomyces cerevisiae Proteins
RNA Stability
Molecular Sequence Data
Saccharomyces cerevisiae
Ribosome biogenesis
Protein Serine-Threonine Kinases
Biochemistry
chemistry.chemical_compound
Gene interaction
Gene Expression Regulation, Fungal
Amino Acid Sequence
Molecular Biology
Messenger RNA
biology
Glycogen
Intracellular Signaling Peptides and Proteins
Gene Expression Regulation, Developmental
Nuclear Proteins
Translation (biology)
General Medicine
biology.organism_classification
DNA-Binding Proteins
Meiosis
Phenotype
chemistry
Mutation
Transfer RNA
Dual-Specificity Phosphatases
Protein Processing, Post-Translational
Ribosomes
Biogenesis
Transcription Factors
Subjects
Details
- ISSN :
- 0021924X
- Volume :
- 150
- Database :
- OpenAIRE
- Journal :
- Journal of Biochemistry
- Accession number :
- edsair.doi.dedup.....cb577cd4fa02b56fd5a8d175a6a885f9