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Ultrasound-Assisted Liposuction Does Not Compromise the Regenerative Potential of Adipose-Derived Stem Cells
- Source :
- Stem Cells Translational Medicine
- Publication Year :
- 2015
- Publisher :
- Oxford University Press (OUP), 2015.
-
Abstract
- The regenerative abilities of adipose-derived mesenchymal stem cells (ASCs) harvested via a third-generation ultrasound-assisted liposuction (UAL) device versus ASCs obtained via standard suction-assisted lipoaspiration were evaluated. ASC yield and viability, and expression of most osteogenic, adipogenic, and key regenerative genes were equivalent between the two methods. Cells harvested via UAL showed comparable abilities to enhance cutaneous regeneration and appear suitable for cell therapy and tissue engineering applications.<br />Human mesenchymal stem cells (MSCs) have recently become a focus of regenerative medicine, both for their multilineage differentiation capacity and their excretion of proregenerative cytokines. Adipose-derived mesenchymal stem cells (ASCs) are of particular interest because of their abundance in fat tissue and the ease of harvest via liposuction. However, little is known about the impact of different liposuction methods on the functionality of ASCs. Here we evaluate the regenerative abilities of ASCs harvested via a third-generation ultrasound-assisted liposuction (UAL) device versus ASCs obtained via standard suction-assisted lipoaspiration (SAL). Lipoaspirates were sorted using fluorescent assisted cell sorting based on an established surface-marker profile (CD34+/CD31−/CD45−), to obtain viable ASCs. Yield and viability were compared and the differentiation capacities of the ASCs were assessed. Finally, the regenerative potential of ASCs was examined using an in vivo model of tissue regeneration. UAL- and SAL-derived samples demonstrated equivalent ASC yield and viability, and UAL ASCs were not impaired in their osteogenic, adipogenic, or chondrogenic differentiation capacity. Equally, quantitative real-time polymerase chain reaction showed comparable expression of most osteogenic, adipogenic, and key regenerative genes between both ASC groups. Cutaneous regeneration and neovascularization were significantly enhanced in mice treated with ASCs obtained by either UAL or SAL compared with controls, but there were no significant differences in healing between cell-therapy groups. We conclude that UAL is a successful method of obtaining fully functional ASCs for regenerative medicine purposes. Cells harvested with this alternative approach to liposuction are suitable for cell therapy and tissue engineering applications. Significance Adipose-derived mesenchymal stem cells (ASCs) are an appealing source of therapeutic progenitor cells because of their multipotency, diverse cytokine profile, and ease of harvest via liposuction. Alternative approaches to classical suction-assisted liposuction are gaining popularity; however, little evidence exists regarding the impact of different liposuction methods on the regenerative functionality of ASCs. Human ASC characteristics and regenerative capacity were assessed when harvested via ultrasound-assisted (UAL) versus standard suction-assisted liposuction. ASCs obtained via UAL were of equal quality when directly compared with the current gold standard harvest method. UAL is an adjunctive source of fully functional mesenchymal stem cells for applications in basic research and clinical therapy.
- Subjects :
- Adipose-derived stem cells
Male
0301 basic medicine
medicine.medical_treatment
Adult mesenchymal stem cells
Regenerative medicine
Cell therapy
Mice
0302 clinical medicine
Tissue engineering
Adipocytes
Ultrasonography
Adipogenesis
Cell Differentiation
General Medicine
Middle Aged
Flow Cytometry
3. Good health
Cell biology
Elective Surgical Procedures
030220 oncology & carcinogenesis
Intercellular Signaling Peptides and Proteins
Female
Adult
medicine.medical_specialty
Cell Survival
Abdominal Fat
Mice, Nude
Neovascularization, Physiologic
03 medical and health sciences
Chondrocytes
Lipectomy
Antigens, CD
Tissue Engineering and Regenerative Medicine
medicine
Animals
Humans
Progenitor cell
Fat harvest
Wound Healing
Osteoblasts
business.industry
Regeneration (biology)
Mesenchymal stem cell
Mesenchymal Stem Cells
Cell Biology
Surgery
030104 developmental biology
Liposuction
Ultrasound-assisted liposuction
Wound healing
business
Biomarkers
Developmental Biology
Subjects
Details
- ISSN :
- 21576580 and 21576564
- Volume :
- 5
- Database :
- OpenAIRE
- Journal :
- Stem Cells Translational Medicine
- Accession number :
- edsair.doi.dedup.....cb492c3f5416b436ff5d5371a0303a33