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Biotechnological Processing of Laying Hen Paw Collagen into Gelatins

Authors :
Jana Pavlačková
Robert Gál
Jiří Mlček
Ngo Thi Hong Linh
Pavel Mokrejs
Source :
Processes, Vol 8, Iss 1415, p 1415 (2020), Processes, Volume 8, Issue 11
Publication Year :
2020
Publisher :
MDPI AG, 2020.

Abstract

By-products of laying hens represent a promising raw material source with a high collagen content, which is currently not adequately used. The aim of the paper is to prepare gelatins from laying hen paws. The purified collagen raw material was processed by a biotechnological process using the food endoprotease Protamex&reg<br />After cleavage of the cross-links in the collagen structure, the gelatin was extracted by a batch process with a stirrer in two extraction steps. The influence of the extraction process on the yield of gelatins and on selected qualitative parameters of gelatins was monitored by two-level factor experiments with three selected process factors. The studied factors were: enzyme dosage (0.2&ndash<br />0.8%), enzyme processing time (24&ndash<br />72 h) and gelatin extraction time (30&ndash<br />120 min). After the first extraction step at 75 &deg<br />C, gelatin was extracted with a yield of 8.2&ndash<br />21.4% and a gel strength of 275&ndash<br />380 Bloom. In the second extraction step at 80&ndash<br />100 &deg<br />C, it is possible to obtain another portion (3.3&ndash<br />7.7%) of gelatin with a gel strength of 185&ndash<br />273 Bloom. Total extraction efficiency of gelatins prepared from laying hen collagen is almost 30%. The prepared gelatins are of high quality and, under proper extraction conditions, gelatins with a gel strength above 300 Bloom can be prepared, thus equaling commercial beef and pork gelatins of the highest quality. Biotechnological processing of laying hen collagen into gelatins is environmentally friendly.

Details

Language :
English
ISSN :
22279717
Volume :
8
Issue :
1415
Database :
OpenAIRE
Journal :
Processes
Accession number :
edsair.doi.dedup.....cb48b4711aedaf322b58efa6abb38955