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Quantitative in situ time-series evaluation of osteoblastic collagen synthesis under cyclic strain using second-harmonic-generation microscopy
- Source :
- Journal of Biomedical Optics
- Publication Year :
- 2019
- Publisher :
- SPIE-Intl Soc Optical Eng, 2019.
-
Abstract
- The aim of this study is to evaluate the osteoblastic collagen synthesis under mechanical stimulation using second-harmonic-generation (SHG) microscopy. We apply SHG microscopy to monitor the collagen fibers synthesized by osteoblast-like cells (MC3T3-E1) without the need for fixation and staining. To quantitatively evaluate the influence of mechanical stimulation on osteoblastic collagen synthesis, we compare SHG images of osteoblast-synthesized collagen fibers with and without a cyclic stretch stimulus applied using a lab-made stretching device. We acquire SHG images every 7 days for 3 weeks at different stimulus conditions (5 min/day and 3 h/day with a strain magnitude of 5% and a frequency of 0.5 Hz). Image analysis of the average SHG intensity indicates that the amount of osteoblastic collagen synthesis is significantly enhanced by the cyclic stretch compared with the nonstretched condition, while there is no significant difference between the two mechanical stimulation conditions. Furthermore, the maturity of the collagen fibers in the early stage of bone formation is not affected by the mechanical stimulation. The results can be used in bone regenerative medicine to apply feedback control of collagen synthesis by artificial stimulation.
- Subjects :
- Paper
collagen
In situ
Cyclic strain
animal structures
Materials science
Biomedical Engineering
Stimulation
01 natural sciences
010309 optics
Biomaterials
Image Interpretation, Computer-Assisted
0103 physical sciences
Microscopy
medicine
Humans
Bone formation
mechanical stimulation
Chemistry
Special Section on Biomedical Imaging and Sensing
Second-harmonic generation
second-harmonic-generation
Osteoblast
Second Harmonic Generation Microscopy
Atomic and Molecular Physics, and Optics
Electronic, Optical and Magnetic Materials
medicine.anatomical_structure
Biophysics
osteoblast
Stress, Mechanical
bone regenerative medicine
Biomedical engineering
Subjects
Details
- ISSN :
- 10833668
- Volume :
- 24
- Database :
- OpenAIRE
- Journal :
- Journal of Biomedical Optics
- Accession number :
- edsair.doi.dedup.....cb36076ee3512b2e774f3621459476be